Journal ArticleDOI
A multitechnique study of liposome adsorption on Au and lipid bilayer formation on SiO2.
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TLDR
The combination of SPR and QCM-D, complemented by atomic force microscopy measurements, has enabled a complete, time-resolved separation of vesicle and bilayer coverages, and thus, for the first time, allowed precise quantification of the critical surface coverage ofvesicles needed for rupture.Abstract:
We have used a new setup for parallel quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance (SPR) measurements to measure the detailed kinetics of vesicle-to-bilayer transformation on SiO2 and vesicle adsorption on Au, respectively. The combination of SPR and QCM-D, complemented by atomic force microscopy measurements, has enabled a complete, time-resolved separation of vesicle and bilayer coverages, and thus, for the first time, allowed precise quantification of the critical surface coverage of vesicles needed for rupture. We furthermore demonstrate and quantify a previously undetected vesicle-size- and concentration-dependent loss of lipid material during the later stages of the process.read more
Citations
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Surface acoustic wave sensors in the bioanalytical field: Recent trends and challenges
TL;DR: This is a comparison of the latest developments in the emerging field of surface acoustic wave (SAW) sensors, shown to be extremely useful for the analysis of both small and large molecules as well as whole cells interacting with an immobilized binding partner.
Journal ArticleDOI
Membrane biosensor platforms using nano- and microporous supports.
Erik Reimhult,Karthik Kumar +1 more
TL;DR: Improved techniques for lipid membrane self-assembly and membrane protein incorporation on nano- and microporous substrates have led to great improvements in measurement sensitivity, membrane stability and packaging, suggesting that nanopore-spanning membranes are leading contenders for a breakthrough in membrane protein screening and biosensing applications.
Journal ArticleDOI
Nanoplasmonic sensors for biointerfacial science
TL;DR: The goal of this review is to introduce the latest advances in nanoplasmonic sensors for the biointerfacial sciences, including ongoing development of nanoparticle and nanohole arrays for exploring different classes of biomacromolecules interacting at solid-liquid interfaces.
Journal ArticleDOI
Recent advances in surface plasmon resonance based techniques for bioanalysis
K. Scott Phillips,Quan Cheng +1 more
TL;DR: An updated 2006 review of SPR, SPR spectroscopy, and SPR imaging explores cutting-edge technology with a focus on material, method, and instrument development and considers the future outlook for SPR and SPR-associated BIA studies.
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A survey of the 2006–2009 quartz crystal microbalance biosensor literature
Bernd Becker,Mark E. Cooper +1 more
TL;DR: This paper highlights theoretical and practical aspects of the principles that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and membrane interfaces.
References
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Journal ArticleDOI
Supported Membranes: Scientific and Practical Applications
TL;DR: Supporting lipid-protein bilayers form versatile models of low-dimensionality complex fluids, which can be used to study interfacial forces and wetting phenomena, and enable the design of phantom cells to explore the interplay of lock-and-key forces and universal forces for cell adhesion.
Journal ArticleDOI
Configurations of fluid membranes and vesicles
TL;DR: In this article, the authors describe the systematic physical theory developed to understand the static and dynamic aspects of membrane and vesicle configurations, and the preferred shapes arise from a competition between curvature energy which derives from the bending elasticity of the membrane, geometrical constraints such as fixed surface area and fixed enclosed volume, and a signature of the bilayer aspect.
Journal ArticleDOI
Viscoelastic Acoustic Response of Layered Polymer Films at Fluid-Solid Interfaces: Continuum Mechanics Approach
TL;DR: In this article, the authors derived the general solution of a wave equation describing the dynamics of two-layer viscoelastic polymer materials of arbitrary thickness deposited on solid (quartz) surfaces in a fluid environment.
Journal ArticleDOI
Quartz crystal microbalance setup for frequency and Q‐factor measurements in gaseous and liquid environments
TL;DR: In this paper, an experimental setup has been constructed for simultaneous measurements of the frequency, the absolute Q factor, and the amplitude of oscillation of a quartz crystal microbalance (QCM).
Journal ArticleDOI
Variations in Coupled Water, Viscoelastic Properties, and Film Thickness of a Mefp-1 Protein Film during Adsorption and Cross-Linking: A Quartz Crystal Microbalance with Dissipation Monitoring, Ellipsometry, and Surface Plasmon Resonance Study
TL;DR: It is shown how quantitative data about the thickness, shear elastic modulus, and shear viscosity of the protein film can be obtained with the QCM-D technique, even beyond the Sauerbrey regime, if frequency (f) and energy dissipation (D) measurements measured at multiple harmonics are combined with theoretical simulations using a Voight-based viscoelastic model.
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Surface specific kinetics of lipid vesicle adsorption measured with a quartz crystal microbalance.
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