Proceedings ArticleDOI
A novel 'Gold on Gold' biosensing scheme for an on-fiber immunoassay
TLDR
A novel „gold on gold” biosensing scheme for absorbance based fiber-optic biosensor that demonstrated the potential in improving the limit of detection for the fiber- optic biosensors and its response was found to be more than five-fold higher than the control bioassay.Abstract:
In this paper, we propose a novel „gold on gold‟ biosensing scheme for absorbance based fiber-optic biosensor. First, a self-assembled monolayer of gold nanoparticles is formed at the sensing region of the fiber-optic probe by incubating an amino-silanized probe in a colloidal gold solution. Thereafter, the receptor moieties, i.e. Human immunoglobulin G (HIgG) were immobilized by using standard alkanethiol and classic carbodiimide coupling chemistry. Finally, biosensing experiments were performed with different concentrations of gold nanoparticle-tagged analyte, i.e. Goat anti- Human immunoglobulin G (Nanogold-GaHIgG). The sensor response was observed to be more than five-fold compared to the control bioassay, in which the sensor matrix was devoid of gold nanoparticle film. Also, the response was found to be ~10 times higher compared to the FITC-tagged scheme and ~14.5 times better compared to untagged scheme. This novel scheme also demonstrated the potential in improving the limit of detection for the fiber-optic biosensors.read more
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Journal ArticleDOI
Detection and differentiation of α-Synuclein monomer and fibril by chitosan film coated nanogold array on optical sensor platform
TL;DR: A new platform for sensitive detection of amyloid fibrils and monomer of α-Synuclein (independently as well as in mixtures) for potential applications in inhibition and aggregation based studies is introduced.
Journal ArticleDOI
Trends in the Design of Intensity-Based Optical Fiber Biosensors (2010–2020)
TL;DR: In this article, a review of optical fiber biosensors based on luminescence and absorption is presented, where a comprehensive insight of the different aspects that must be taken into account when working with this kind of sensors is provided.
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Inexpensive Polyester Sheet Based Waveguides for Detection of Cardiac Biomarker, Myeloperoxidase
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Journal ArticleDOI
Tailored Aggregate-Free Au Nanoparticle Decorations with Sharp Plasmonic Peaks on a U-Type Optical Fiber Sensor by Nanosecond Laser Irradiation
D. Spasopoulos,S. Kaziannis,Alexander E. Karantzalis,Elefterios Lidorikis,A. Ikiades,Constantine Kosmidis +5 more
TL;DR: In this article, the U-shaped optical fiber probes were fabricated with aggregate-free Au nanoparticles exhibiting a sharp localized surface plasmon resonance (LSPR) spectra, and the process was monitored and controlled in real time through the changes induced into the fiber's extinction spectra by the laser irradiation.
References
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Journal ArticleDOI
A study of the nucleation and growth processes in the synthesis of colloidal gold
TL;DR: In this paper, a preliminary survey with the electron microscope of various preparations of colloidal gold, a study was made of the process of nucleation and growth in gold colloids, and it was shown that nucleating agents may be identified with reducing agents which form a mixed polymer with chlorauric ion before the reduction to the nucleus takes place.
Journal ArticleDOI
LSPR-based nanobiosensors
TL;DR: The basis behind LSPR sensing is described, the latest progress regarding nanostructure fabrication techniques and biosensing applications are summarized, and the challenges that L SPR biosensors should face are discussed.
Journal ArticleDOI
Multiplex biosensor using gold nanorods.
Chenxu Yu,Joseph Irudayaraj +1 more
TL;DR: Multiplex sensing was demonstrated by the distinct response of the plasmon spectra of the GNrMPs to binding events of three targets, which can play a key role in developing novel optical biosensors for both in vivo and in vitro detection and single-receptor kinetics.
Journal ArticleDOI
Gold nanoparticle-based biosensors
TL;DR: In this paper, a review of the recent advances in the development of gold nanoparticles-based optical, electrochemical, and piezoelectric biosensors is presented.
Journal ArticleDOI
Novel U-bent fiber optic probe for localized surface plasmon resonance based biosensor.
TL;DR: A sensor for sensitive measurement of refractive index (RI) with the help of optical absorbance properties of gold nanoparticles (GNP) coupled to an efficient optical transducer in the form of a U-bent fiber optic probe is described.