A rhamnose-binding lectin from Rhodnius prolixus and the impact of its silencing on gut bacterial microbiota and Trypanosoma cruzi.
C.A.C. Araújo,João Pacheco,P.J. Waniek,Reinaldo B Geraldo,A. Sibajev,A.L. Dos Santos,Victor G O Evangelho,Paul Dyson,Patrícia Azambuja,Norman A. Ratcliffe,Helena Carla Castro,Cicero B. Mello +11 more
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TLDR
The results with RpLec reveal the potential importance of this little studied molecule in the insect vector immune response and homeostasis of the gut bacterial microbiota.Abstract:
Lectins are ubiquitous proteins involved in the immune defenses of different organisms and mainly responsible for non-self-recognition and agglutination reactions. This work describes molecular and biological characterization of a rhamnose-binding lectin (RBL) from Rhodnius prolixus, which possesses a 21 amino acid signal peptide and a mature protein of 34.6 kDa. The in-silico analysis of the primary and secondary structures of RpLec revealed a lectin domain fully conserved among previous insects studied. The three-dimensional homology model of RpLec was similar to other RBL-lectins. Docking predictions with the monosaccharides showed rhamnose and galactose-binding sites comparable to Latrophilin-1 and N-Acetylgalactosamine-binding in a different site. The effects of RpLec gene silencing on levels of infecting Trypanosoma cruzi Dm 28c and intestinal bacterial populations in the R. prolixus midgut were studied by injecting RpLec dsRNA into the R. prolixus hemocoel. Whereas T. cruzi numbers remained unchanged compared with the controls, numbers of bacteria increased significantly. The silencing also induced the up regulation of the R. prolixus defC (defensin) expression gene. These results with RpLec reveal the potential importance of this little studied molecule in the insect vector immune response and homeostasis of the gut bacterial microbiota.read more
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RNAi-mediated silencing of an egg-specific gene Nllet1 results in hatch failure in the brown planthopper.
Jia-Bao Lu,Sai-nan Wang,Peng-Peng Ren,Fang He,Qiao Li,Jian-Ping Chen,Junmin Li,Chuan-Xi Zhang +7 more
TL;DR: This study indicates that Nllet1 is a promising candidate gene that can be used to develop RNAi-based control strategies at the BPH egg stage, and it can also be used as a target for developing novel ovicides.
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A novel l-rhamnose-binding lectin participates in defending against bacterial infection in zebrafish.
TL;DR: In this article , the authors discovered a new l-rhamnose binding lectin (DrRBL) and studied its innate immune function, which is a class of animal lectins independent of Ca2+, can specifically bind l-RHAMnose or d-galactose.
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Digestive α-L-fucosidase activity in Rhodnius prolixus after blood feeding: effect of secretagogue and nutritional stimuli
TL;DR: In this paper , it was shown that the enzyme α-L-fucosidase was produced in the anterior midgut of R. prolixus after feeding with the plasma fraction.
Journal ArticleDOI
New Data on the Rhamnose-Binding Lectin from the Colonial Ascidian Botryllus schlosseri: Subcellular Distribution, Secretion Mode and Effects on the Cyclical Generation Change
Giacomo Bovo,Loriano Ballarin +1 more
TL;DR: In this paper , the authors studied the subcellular distribution of BsRBL during immune responses using light and electron microscopy and investigated the effects of interfering with this protein, by injecting a specific antibody in the colonial circulation, starting one day before the generation change.
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A role of peptidoglycan recognition protein in mediating insecticide detoxification in Glyphodes pyloalis.
De-Lei Jiang,Jian-Hao Ding,Zhi-xiang Liu,Zuo-Ming Shao,Xin-hao Liang,Jun Wang,Fu-An Wu,Sheng Sheng +7 more
TL;DR: Zhang et al. as mentioned in this paper investigated the possible detoxication function of Peptidoglycan recognition proteins (PGRPs) in G. pyloalis in response to chlorfenapyr and phoxim.
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