A role for exopolysaccharides in the protection of microorganisms from desiccation.
Tslil Ophir,David L. Gutnick +1 more
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TLDR
Mucoid strains of Escherichia coli, Acinetobacter calcoaceticus, and Erwinia stewartii were significantly more resistant to desiccation than corresponding isogenic nonmucoid mutants, even in colonies containing both cell types.Abstract:
Mucoid strains of Escherichia coli, Acinetobacter calcoaceticus, and Erwinia stewartii were significantly more resistant to desiccation than corresponding isogenic nonmucoid mutants (survival rates of up to 35% in mucoid strains and between 0.7 and 5% in nonmucoid variants), even in colonies containing both cell types. Desiccation was found to bring about an induction of beta-galactosidase in Lon strains of E. coli K-12 carrying transcriptional lac fusions in the capsule biosynthetic (cps) regulon. This induction was dependent on the transcriptional activators RcsA and RcsB. Induction was lower in cells carrying mutations in the membrane sensor protein RcsC.read more
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The biochemistry and genetics of capsular polysaccharide production in bacteria
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References
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Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
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Relationship between Desiccation and Exopolysaccharide Production in a Soil Pseudomonas sp
TL;DR: It is concluded that bacteria may use EPS production to alter their microenvironment to enhance survival of desiccation, and an EPS matrix may buffer bacterial colonies from some effects of Desiccation.
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Exopolysaccharides in Plant-Bacterial Interactions
John A. Leigh,David L. Coplin +1 more
TL;DR: In Rhizobium species, exopolysaccharide synthesis is regulated by transcriptional as well as posttranslational mechanisms, and Regulatory genes that have homology to the two-component sensor and transcriptional effector systems are a common motif.
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Transformation of Acinetobacter calco-aceticus (Bacterium anitratum).
Elliot Juni,Alice Janik +1 more
TL;DR: The finding of transformation in Acinetobacter may provide an additional useful organism for the study of this mode of genetic transfer since this strain grows well in a simple mineral medium containing a single oxidizable source of carbon.
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Regulation of capsular polysaccharide synthesis in Escherichia coli K-12: characterization of three regulatory genes.
TL;DR: In this paper, mutations in three new regulatory genes (rcs, for regulator of capsule synthesis) that control expression of these same fusions were identified and characterized, and mapped to the E. coli map.