Journal ArticleDOI
A simple method for DNA purification from peripheral blood.
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TLDR
A new, simple, and inexpensive method for the rapid isolation of DNA from whole blood is described, thus facilitating gene linkage or other molecular studies in which large numbers of individuals are required.About:
This article is published in Analytical Biochemistry.The article was published on 1988-11-01. It has received 247 citations till now. The article focuses on the topics: DNA extraction & DNA.read more
Citations
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Journal ArticleDOI
Rapid and simple method for purification of nucleic acids.
R. Boom,C. J. A. Sol,M. M. M. Salimans,C. L. Jansen,P.M.E. Wertheim-van Dillen,J. van der Noordaa +5 more
TL;DR: A simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine, based on the lysing and nuclease-inactivating properties of guanidinium thiocyanate together with the nucleic acid-binding properties of silica particles or diatoms in the presence of this agent.
Journal ArticleDOI
Oxidative DNA damage in prostate cancer patients consuming tomato sauce-based entrees as a whole-food intervention.
Longwen Chen,Maria Stacewicz-Sapuntzakis,Claudine Duncan,Roohollah Sharifi,Luna Ghosh,Richard B. van Breemen,David Ashton,Phyllis E. Bowen +7 more
TL;DR: A possible role for a tomato sauce constituent, possibly lycopene, in the treatment of prostate cancer is indicated and warrant further testing with a larger sample of patients, including a control group.
Journal ArticleDOI
Whole Genome Analysis of Genetic Alterations in Small DNA Samples Using Hyperbranched Strand Displacement Amplification and Array–CGH
José M. Lage,John H. Leamon,Tanja Pejovic,Stefan Hamann,Michelle Lacey,Deborah A. Dillon,Richard Segraves,Bettina Vossbrinck,Antonio González,Daniel Pinkel,Donna G. Albertson,Jose Costa,Paul M. Lizardi +12 more
TL;DR: The whole genome isothermal amplification is described, in combination with comparative genomic hybridization on cDNA microarrays, and the ability to detect gene losses in yeast and gene dosage imbalances in human breast tumor cell lines is demonstrated.
Journal ArticleDOI
Mutation of MYH9, encoding non-muscle myosin heavy chain A, in May-Hegglin anomaly.
TL;DR: The identification of MYH9 as the disease gene for MHA establishes the pathogenesis of the disorder, should provide further insight into the processes of normal platelet formation and may facilitate identification of the genetic basis of related disorders.
BookDOI
Nutritional aspects of osteoporosis
TL;DR: Part I Nutrition and development of bone in childhood and adolescence: calcium intake and age influence calcium retention in adolescence and the effect of enhanced bone gain archived with calcium supplementation during ages 12-16 does not persist in late adolescence.
References
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Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
A general method for isolation of high molecular weight DNA from eukaryotes
Nikolaus Blin,Darrel W. Stafford +1 more
TL;DR: A new method for isolation of high molecular weight DNA from eukaryotes is presented, which allows preparation of DNA from a variety of tissues and from cells which were more difficult to lyse until now.
Journal ArticleDOI
Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants.
L. M. Kunkel,Kirby D. Smith,Samuel H. Boyer,D S Borgaonkar,Stephen S. Wachtel,O.J. Miller,W. R. Breg,H W Jones,J M Rary +8 more
TL;DR: Correlation with phenotype and other known Y chromosome markers establish that the Y-chromosome-specific reiterated DNA discussed here has no evident role in male determination.
Analysis of human Y-chromosome-specific reiterated DNA in
L. M. Kunkel,Kirby D. Smith,Samuel H. Boye,Digamber S. Borgaonkar,W. Roy Breg,Howard W. Jones,Jack M. Raryt +6 more
TL;DR: A number of individuals with aberrant Y chromosomes have been tested for the presence of Y-chromo-some-specific reiterated DNA as discussed by the authors, which represents at least 10% of Y chromosome DNA.