A stable genomic source of P element transposase in Drosophila melanogaster.
Hugh M. Robertson,Christine R. Preston,Randall W. Phillis,Dena M. Johnson-Schlitz,Wendy K. Benz,William R. Engels +5 more
TLDR
A single P element insert in Drosophila melanogaster, called P[ry+ delta 2-3](99B), is described that caused mobilization of other elements at unusually high frequencies, yet is itself remarkably stable.Abstract:
A single P element insert in Drosophila melanogaster, called P[ry+ delta 2-3](99B), is described that caused mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for "helper" plasmids in P element mediated transformation, and can be used to move transformed elements around the genome.read more
Citations
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Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
TL;DR: The GAL4 system, a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns, has been designed and used to expand the domain of embryonic expression of the homeobox protein even-skipped.
Journal ArticleDOI
An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases
TL;DR: The efficiency, ease-of-use, and versatility obtained here with the φC31-based integration system represents an important advance in transgenesis and opens up the possibility of systematic, high-throughput screening of large cDNA sets and regulatory elements.
Journal ArticleDOI
Extension of life-span by overexpression of superoxide dismutase and catalase in Drosophila melanogaster
William C. Orr,Rajindar S. Sohal +1 more
TL;DR: A study of the effects of simultaneous overexpression of copper-zinc superoxide dismutase and catalase provides direct support for the free radical hypothesis of aging.
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Armadillo Coactivates Transcription Driven by the Product of the Drosophila Segment Polarity Gene dTCF
Marc van de Wetering,Robert Cavallo,Dennis Dooijes,Moniek van Beest,Johan H. van Es,Joseph Loureiro,Arne Ypma,Deborah A. Hursh,Tamara L. Jones,Amy Bejsovec,Mark Peifer,Mark A. Mortin,Hans Clevers +12 more
TL;DR: A maternally expressed Drosophila TCF family member is cloned, dTCF, which mediates Wingless signaling as a bipartite transcription factor and binds a canonical TCF DNA motif and interacts with the beta-catenin homolog Armadillo.
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Genetic control of programmed cell death in Drosophila
Kristin White,Megan E. Grether,John M. Abrams,Lynn Young,Lynn Young,Kim Farrell,Hermann Steller +6 more
TL;DR: A gene, reaper (rpr), that appears to play a central control function for the initiation of programmed cell death (apoptosis) in Drosophila was identified and suggests that the basic cell death program is intact although it was not activated in mutant embryos.
References
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Genetic Transformation of Drosophila with Transposable Element Vectors
TL;DR: A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected rosy mutant embryos indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene.
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Transposition of cloned P elements into Drosophila germ line chromosomes
TL;DR: Recombinant DNA carrying the 3-kilobase transposable element was injected into Drosophila embryos of a strain that lacked such elements, and observations provide the basis for efficient DNA-mediated gene transfer in Dosophila.
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Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome
Kevin O'Hare,Gerald M. Rubin +1 more
TL;DR: A revertant of one of the white locus mutants has been found to result from precise excision of the P element, restoring the wild-type DNA sequence.
Journal ArticleDOI
Analysis of P transposable element functions in drosophila
Roger E. Karess,Gerald M. Rubin +1 more
TL;DR: A P-element derivative called Pc[ry], which carries the selectable marker gene rosy, but which acts like a nondefective, intact P element, is made, which suggests that a single polypeptide comprises the transposase.