Journal ArticleDOI
α-Tocopherol modifies tyrosine phosphorylation and capacitation-like state of cryopreserved porcine sperm
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TLDR
The addition of alpha-tocopherol to the extender prior to cryopreservation of boar semen protected sperm membranes against oxidative damage and reduced both tyrosine phosphorylation and the capacitation-like state.About:
This article is published in Theriogenology.The article was published on 2007-10-15. It has received 70 citations till now. The article focuses on the topics: Sperm & Tyrosine phosphorylation.read more
Citations
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Journal ArticleDOI
The effect of antioxidants on motility, viability, acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa.
TL;DR: In conclusion, cysteine or vitamin E supplementation of tris egg yolk extender improved motility, progressive motility and integrity of the sperm membrane and DNA of frozen-thawed epididymal cat spermatozoa.
Journal ArticleDOI
Proteomic characteristics of human sperm cryopreservation.
Shangqian Wang,Wei Wang,Yang Xu,Min Tang,Jianzheng Fang,Hong-yong Sun,Yang-Yang Sun,Mei-Juan Gu,Liu Zhili,Zhang Zhaoxia,Lin Faxi,Ting Wu,Ninghong Song,Zengjun Wang,Wei Zhang,Changjun Yin +15 more
TL;DR: Proteomic analysis of differential protein characteristics between freeze‐thawed and fresh sperm samples obtained from nine normozoospermic donors suggest that dysfunctional spermatozoon after cryopreservation may be due to protein degradation and protein phosphorylation.
Journal ArticleDOI
Anti-oxidant supplementation improves boar sperm characteristics and fertility after cryopreservation: Comparison between cysteine and rosemary (Rosmarinus officinalis)
TL;DR: The addition of exogenous rosemary or cysteine to the freezing extender positively affected post-thawed viability and acrosome integrity and the inclusion of rosemary into the extender was effective in penetration and cleavage rate and also in the efficiency of the fertilization system.
Journal ArticleDOI
Reactive oxygen species generators affect quality parameters and apoptosis markers differently in red deer spermatozoa.
Felipe Martínez-Pastor,E.G. Aisen,María Rocío Fernández-Santos,Milagros C. Esteso,Alejandro Maroto-Morales,Olga García-Álvarez,José Julián Garde +6 more
TL;DR: There were important differences between ROS generators, H( 2)O(2) being the most cytotoxic and caspase activation was tested using the (FAM-VAD-FMK) probe.
Journal ArticleDOI
Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L.
Ping Li,Ping Li,Martin Hulak,P. Koubek,Miroslav Sulc,Borys Dzyuba,Borys Dzyuba,Sergii Boryshpolets,Marek Rodina,David Gela,P. Manaskova-Postlerova,Jana Peknicova,Otomar Linhart +12 more
TL;DR: It is hypothesize that transferrin in cryopreserved sperm may protect spermatozoa against oxidative damage during the freeze-thaw process.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
The causes of reduced fertility with cryopreserved semen
TL;DR: Evidence for new approaches for improving the performance of cryopreserved semen is offered and factors affecting the proportion of survivors and functional status of survivors are reviewed.
Journal ArticleDOI
Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function.
TL;DR: The hypothesis is advanced that cryopreserved mammalian spermatozoa are in a state resembling capacitation, which accounts for their relatively reduced longevity and their readiness to undergo egg penetration without incubation.
Journal ArticleDOI
Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway.
Pablo E. Visconti,Grace D. Moore,Janice L. Bailey,P. Leclerc,Stephanie A. Connors,Dieyun Pan,Patricia Olds-Clarke,Gregory S. Kopf +7 more
TL;DR: Up-regulation of protein tyrosine phosphorylation by cAMP/PKA in sperm is, to the authors' knowledge, the first demonstration of such an interrelationship between tyrosin kinase/phosphatase and PKA signaling pathways.
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Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing.
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