Journal ArticleDOI
Abscisic acid and gibberellic acid‐regulated responses of embryos and aleurone layers isolated from dormant and nondormant barley grains
TLDR
Embryos and aleurone layers from dormant grains responded stronger to ABA than those from nondormant grains and counteracted the ABA-induced inhibition of embryo germination.Abstract:
Dormant and nondormant isogenic barley grains were obtained by maturing grains under short day (SD) or long day (LD) growth conditions, respectively. Hormonal responses of isolated embryos and aleurone layers from these grains were studied. Addition of abscisic acid (ABA) reduced germination rate and percentage of embryos, and induced Rab (ABA-responsive) mRNA in aleurone layers from both types of grain. Embryos and aleurone layers from dormant grains responded stronger to ABA than those from nondormant grains. Gibberellic acid (GA 3 ) increased the germination rate and percentage of embryos from dormant grains and counteracted the ABA-induced inhibition of embryo germinationread more
Citations
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Journal ArticleDOI
Sugar Repression of a Gibberellin-Dependent Signaling Pathway in Barley Embryos.
TL;DR: The results show that sugar and hormonal signaling interact in the regulation of gibberellic acid-induced gene expression in barley grains, suggesting the existence of an interaction between the hormonal and metabolic regulation of [alpha]-amylase genes in barley grain.
Journal ArticleDOI
Apoptosis in barley aleurone during germination and its inhibition by abscisic acid
TL;DR: It is hypothesized that the regulation of DNA fragmentation in aleurone plays a very important role in spatial and temporal control of aleur one activities during germination and during protoplast isolation.
cGMP 1s Required for Gibberellic Acid-lnduced Gene Expression in Barley Aleurone
Simon P. Penson,Robert C. Schuurink,Angelika Fath,Frank Gubler,John V. Jacobsen,Russell L. Jones +5 more
TL;DR: It is concluded that cGMP plays an important role in GA, but not ABA, signaling in the barley aleurone cell.
Journal ArticleDOI
A peroxiredoxin antioxidant is encoded by a dormancy-related gene, Per1, expressed during late development in the aleurone and embryo of barley grains
TL;DR: The data show that the barley seed peroxiredoxin is encoded by a singlePer1 gene, the first antioxidant belonging to the 1-Cys subgroup shown to be functionally active, and the first perxiredoxin of any kind to be functional described in plants.
Journal ArticleDOI
Modulation of germination of embryos isolated from dormant and nondormant barley grains by manipulation of endogenous abscisic acid
TL;DR: The results demonstrate that diffusion of endogenous ABA, de-novo ABA synthesis and ABA sensitivity play a role in the control of germination and it is proposed that dormancy-breaking treatments act via changes to these processes.
References
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Journal ArticleDOI
Gene expression in response to abscisic acid and osmotic stress.
Karen Skriver,John Mundy +1 more
TL;DR: Several ABA-responsive genes have been isolated and a major goal of the research discussed below is to understand the role these genes play in osmotic stress and desiccation tolerance.
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The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana
TL;DR: Three mutants of Arabidopsis thaliana (L.) Heynh were characterized by a reduced seed dormancy and by symptoms of withering, indicating disturbed water relations and resembled phenotypically the ABA-deficient mutants the authors described earlier in this species.
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Common amino acid sequence domains among the LEA proteins of higher plants.
Leon Dure,Martha L. Crouch,John J. Harada,Tuan-Hua David Ho,John Mundy,Ralph S. Quatrano,Terry L. Thomas,Zinmay Renee Sung +7 more
TL;DR: Analysis of conserved tracts of amino acid sequence among LEA proteins from several species suggests that it exists as an amphiphilic α helix which may serve as the basis for higher order structure.
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A plant leucine zipper protein that recognizes an abscisic acid response element
TL;DR: The deduced amino acid sequence of EmBP-1 contains conserved basic and leucine zipper domains found in transcription factors in plants, yeast, and mammals and may be a member of a highly conserved family of proteins that recognize a core sequence found in the regulatory regions of various genes that are integrated into a number of different response pathways.
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Induction of dormancy during seed development by endogenous abscisic acid: studies on abscisic acid deficient genotypes of Arabidopsis thaliana (L.) Heynh.
TL;DR: Mutant lines of Arabidopsis thaliana (L.) Heynh, which are characterized by symptoms of withering and the absence of seed dormancy, showed much lower levels of endogenous abscisic acid (ABA) in developing seeds and fruits than the wild type, and it seems that limited access to water is the primary trigger for the developmental arrest in these seeds.