Journal ArticleDOI
Acoustic cell filter for high density perfusion culture of hybridoma cells
Reads0
Chats0
TLDR
A flow–through device which uses high frequency, low energy ultrasonic resonance fields to transiently aggregate hybridoma cells and return them by sedimentation to a perfusion bioreactor retained up to 99 percent of the inflowing viable cells with no measurable effect on viability.Abstract:
We have developed a flow–through device which uses high frequency, low energy ultrasonic resonance fields to transiently aggregate hybridoma cells and return them by sedimentation to a perfusion bioreactor. The system retained up to 99 percent of the inflowing viable cells with no measurable effect on viability. Viable cells were selectively retained at up to 3 percent higher efficiency than nonviable cells. A stirred tank bioreactor was operated for 700 hours with acoustic cell recycle. Concentrations greater than 5×107 cells/ml were attained with a 5–fold increase in antibody concentration and a 70–fold increase in volumetric productivity compared with batch culture.read more
Citations
More filters
Journal ArticleDOI
Chip integrated strategies for acoustic separation and manipulation of cells and particles
TL;DR: This tutorial review outlines the fundamental work performed on continuous flow acoustic standing wave separation of particles in macro scale systems and discusses several potential applications in life science research and in the medical clinic.
Journal ArticleDOI
Ultrasound, cavitation bubbles and their interaction with cells.
Junru Wu,Wesley L. Nyborg +1 more
TL;DR: The basic physics of ultrasound generation, acoustic field, and both inertial and non-inertial acoustic cavitation in the context of localized gene and drug delivery as well as non-linear oscillation of an encapsulated microbubble and its associated microstreaming and radiation force generated by ultrasound are reviewed.
Journal ArticleDOI
Ultrasound, a new separation technique to harvest microalgae
TL;DR: In this paper, it is proven that ultrasound can be used to harvest microalgae and the efficiency of harvesting and the concentration factor of the ingoing biomass concentration are optimized and the relevance of this process compared to other harvesting processes is determined.
Journal ArticleDOI
Induction of apoptosis in nutrient‐deprived cultures of hybridoma and myeloma cells
Sylvain Mercille,Bernard Massie +1 more
TL;DR: It is concluded that D5 hybridomas and NS/0 myelomas deprived of essential nutrients die by apoptosis, whereas incubation in the presence of elevated levels of metabolic byproducts such as ammonia and lactate will induce necrotic cell death in these cells.
Journal ArticleDOI
Potential of cell retention techniques for large-scale high-density perfusion culture of suspended mammalian cells.
TL;DR: This analysis shows certain techniques have a limited range of perfusion rate where they can be implemented, and techniques were identified that have shown high perfusion capacity, or have a good potential for scale‐up (acoustic settlers and inclined settlers).
References
More filters
Journal ArticleDOI
Spin Filter Culture: The Propagation of Mammalian Cells in Suspension
TL;DR: A spin filter device has been used for the propagation in vitro of cells of mnouse leukemia L1210 to densities approaching 108 cells per milliliter, providing a more realistic parallel to in vivo drug treatment than is obtained by other culture methods.
Journal ArticleDOI
The large-scale cultivation of mammalian cells.
Joseph Feder,William R. Tolbert +1 more
Journal ArticleDOI
Comparison of cell propagation methods for their effect on monoclonal antibody yield in fermentors
TL;DR: A perfusion culturing method was found to be the most effective for production of McAb and the system reached steady-state conditions in which concentration of live cells, dead cells, glucose, lactic acid, ammonium and McAb were relatively constant.
Journal ArticleDOI
General one‐dimensional treatment of the layered piezoelectric resonator with two electrodes
H. Nowotny,Ewald Benes +1 more
TL;DR: In this paper, a general transfer matrix description of arbitrarily oriented layered piezoelectric structures is presented, which allows the calculation of the electrical admission appearing between the electrodes for any frequency, as well as the determination of the entire resonance frequency spectrum using only simple matrix multiplications.
Journal ArticleDOI
Inclined Sedimentation for Selective Retention of Viable Hybridomas in a Continuous Suspension Bioreactor
TL;DR: Intermediate flow rates through the settler have been found to provide the highest removal of nonviable hybridomas relative to viable hybridoma retention and 3‐and 6‐fold increases, respectively, over the values obtained from a chemostat culture without cell retention.