Journal ArticleDOI
Adhesion and tribological properties of hydrophobin proteins in aqueous lubrication on stainless steel surfaces
Timo J. Hakala,Päivi Laaksonen,Päivi Laaksonen,Vesa Saikko,Tiina Ahlroos,Aino Helle,Riitta Mahlberg,Hendrik Hähl,Karin Jacobs,Petri Kuosmanen,Markus Linder,Kenneth Holmberg +11 more
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TLDR
In this paper, the affinities of two different proteins, HFBI and FpHYD5, on stainless steel and their ability to bind water at the surface by combining quartz crystal microbalance (QCM-D) and ellipsometry were investigated.Abstract:
Macroscale tribological properties of hydrophobin layers bound on stainless steel surfaces were investigated in an aqueous environment. Emphasis was on boundary lubrication because water easily fails in hydrodynamic lubrication due to its low viscosity. We studied the affinities of two different proteins, HFBI and FpHYD5, on stainless steel and their ability to bind water at the surface by combining quartz crystal microbalance (QCM-D) and ellipsometry. Both proteins contained an adhesive hydrophobic domain, but FpHYD5 also had a very strongly hydrating carbohydrate structure attached to it. The lubrication properties of the proteins were studied with two different methods, pin-on-disc (POD) (stainless steel vs. stainless steel) and circular translation pin-on-disc (CTPOD) (UHMWPE vs. stainless steel). It was observed that both hydrophobins could adhere to the stainless steel surface and form highly hydrated layers. Both proteins reduced friction and wear of the sliding contact between two stainless steel surfaces. With UHMWPE against stainless steel, the hydrophobins prevented the polyethylene transfer to the counterface. The lowest coefficient of friction (COF) 0.13 was observed when FpHYD5 hydrophobins were employed in pure water. On the other hand, the lowest wear was observed when FpHYD5 proteins were added in a 50 mM sodium acetate buffer. Increasing the water content and loosening the hydrophobin film structure on the stainless steel surface led to a reduction in friction and wear.read more
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Metal release from stainless steel in biological environments: A review
TL;DR: This review aims to compile the different metal release mechanisms that are relevant for stainless steel when used in different biological settings, including corrosion-induced metal release, dissolution of the surface oxide, friction-inducedMetal release, and their combinations.
Journal ArticleDOI
Structural characterization and tribological evaluation of quince seed mucilage
Timo J. Hakala,Vesa Saikko,Suvi Arola,Suvi Arola,Tiina Ahlroos,Aino Helle,Petri Kuosmanen,Kenneth Holmberg,Markus Linder,Markus Linder,Päivi Laaksonen,Päivi Laaksonen +11 more
TL;DR: In this paper, the authors showed that quince mucilage is a native nanocellulose material with a notable ability to lower friction and showed that the mucilage was able to reduce the coefficient of friction of polyethylene/stainless steel contact.
Journal ArticleDOI
Hydrophobin-coated plates as matrix-assisted laser desorption/ionization sample support for peptide/protein analysis
Sara Longobardi,Alfredo Maria Gravagnuolo,Ilaria Rea,Luca De Stefano,Gennaro Marino,Paola Giardina +5 more
TL;DR: The results demonstrate that Vmh2 coating of MALDI plates allows for a very simple and effective desalting method suitable for development of lab-on-a-plate platforms focused on proteomic applications.
Journal ArticleDOI
Pure Protein Bilayers and Vesicles from Native Fungal Hydrophobins
Hendrik Hähl,Jose Nabor Vargas,Alessandra Griffo,Alessandra Griffo,Päivi Laaksonen,Géza R. Szilvay,Michael Lienemann,Karin Jacobs,Ralf Seemann,Jean-Baptiste Fleury +9 more
TL;DR: Pure protein bilayers and vesicles are formed using the native, fungal hydrophobin HFBI and, depending on the type of core, vesicle in water, oily media, and even in air can be created using microfluidic jetting.
Journal ArticleDOI
Hydrophobin-Based Surface Engineering for Sensitive and Robust Quantification of Yeast Pheromones.
TL;DR: This assay was applied to study the pheromone secretion of diverse yeast strains including a whole-cell biosensor strain of Schizosaccharomyces pombe modulating α-factor secretion in response to an environmental signal, yielding a novel kind of biosensor for the yeast phermone with the lowest limit of detection reported so far.
References
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Verwendung von Schwingquarzen zur Wägung dünner Schichten und zur Mikrowägung
TL;DR: In this paper, eigenfrequenz der Platte infolge Vergroserung der schwingenden Masse is vermessen, so das eine empirische Eichung bei der Schichtwagung mit Schwingquarzen entfallt.
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The use of quarts oscillators for weighing thin layers and for microweighing
G. Sauerbrey,G.Z. Sauerbrey +1 more
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Ellipsometry as a tool to study the adsorption behavior of synthetic and biopolymers at the air–water interface
TL;DR: The application of ellipsometry for the study of the adsorption behavior of proteins and synthetic macromolecules at the air-water interface has been investigated in this article.
Journal ArticleDOI
Fluidity of bound hydration layers.
TL;DR: It is found that the bound water molecules retain a shear fluidity characteristic of the bulk liquid, even when compressed down to films 1.0 ± 0.3 nanometer thick, due to the ready exchange of water molecules within the hydration layers as they rub past each other under strong compression.