Journal ArticleDOI
AFLP based alternatives for the assessment of Distinctness, Uniformity and Stability of sugar beet varieties.
Reads0
Chats0
TLDR
Three approaches for addressing criteria for Distinctness, Uniformity and Stability (DUS) assessment by means of AFLP data are presented and what way the result obtained differs and agrees is discussed.Abstract:
Three approaches for addressing criteria for Distinctness, Uniformity and Stability (DUS) assessment by means of AFLP data are presented. AFLP data were obtained for three consecutive seed deliveries of 15 sugar beet varieties that were under investigation for the official Belgian list (’93, ’94 and ’95). In total, 696 AFLP markers were scored on 1350 plants. As a first approach, a cluster analysis based on Nei’s standard genetic distances between varieties and/or seed deliveries was made. Three major groups put together varieties belonging to corresponding breeding programmes. Statistical procedures, involving bootstrapping and random sampling of subsets of markers, were applied to test the reproducibility of the ordinations and the redundancy present in the data set. In a second approach, the genetic structure inferred by varieties and seed deliveries was submitted to an Analysis of Molecular Variance (AMOVA). Major genetic variation was attributed to individual plant differences within seed deliveries. Differences among seed deliveries seemed to be as important as differences among varieties or breeding programmes. Individual plant data were used for assignment tests. The computation of the assignment was based on the ranking of individual genotypes to one other (based on Jaccard similarity coefficients). The distribution over the accessions for each variety or seed delivery was used to check what group of plants each individual is genetically most similar to. Varieties were classified according to the degree to which the distribution over the different accessions was mainly allocated to their appropriate seed deliveries (from the same variety) or cross- allocated to other varieties. Criteria for DUS-evaluation could be set by each of the approaches; it is discussed in what way the result obtained differs and agrees.read more
Citations
More filters
Journal ArticleDOI
PICcalc: An Online Program to Calculate Polymorphic Information Content for Molecular Genetic Studies
TL;DR: There are two measures of the quality or informativeness of a polymorphism as a genetic marker:heterozygosity (H) and polymorphic information content (PIC).
Journal ArticleDOI
A comparative study of molecular and morphological methods of describing genetic relationships in traditional Ethiopian highland maize
TL;DR: Cluster analysis of morphological and marker distances revealed three groups of maize accessions with distinctive genetic profiles and morphological traits that will be useful for collections, conservation and various breeding programs in the highlands of Ethiopia.
Journal ArticleDOI
Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice
R. K. Singh,Ram Kumar Sharma,Ashok Kumar Singh,Vijay P. Singh,Nirmal Singh,S. P. Tiwari,Trilochan Mohapatra +6 more
TL;DR: The marker-based clustering of the varieties corresponded with the known phenotypic classification, thereby providing confidence in the distinctness established by the mapped STMS markers, and suggested that the set of mapped markers employed in this study could be further used for establishing distinctness of aromatic rice varieties and for studying DUS of the important commercial variety Pusa Basmati 1.
Journal ArticleDOI
Genetic diversity analysis of Mediterranean faba bean (Vicia faba L.) with ISSR markers
TL;DR: The present study supported the suggestion that Mediterranean-type faba beans can be subdivided into at least two different germplasm pools and the groups studied are promising for the production of synthetic varieties.
Journal ArticleDOI
Data analysis for molecular characterization of plant genetic resources
TL;DR: The rationale behind analyses achieved to study genetic relationship among accessions (within and between groups) and to identify accession are discussed, and the adequacy of some analyses and/or parameters for specific purposes are discussed.
References
More filters
Journal ArticleDOI
AFLP: a new technique for DNA fingerprinting.
Pieter Vos,René Cornelis Josephus Hogers,Marjo Bleeker,Martin Reijans,Theo van de Lee,Miranda Hornes,Adrie Friters,Jerina Pot,Johan Paleman,Martin Kuiper,Marc Zabeau +10 more
TL;DR: The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity that allows the specific co-amplification of high numbers of restriction fragments.
Journal ArticleDOI
Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data.
TL;DR: In this article, a framework for the study of molecular variation within a single species is presented, where information on DNA haplotype divergence is incorporated into an analysis of variance format, derived from a matrix of squared-distances among all pairs of haplotypes.
Journal Article
Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data.
Journal ArticleDOI
Estimation of average heterozygosity and genetic distance from a small number of individuals
TL;DR: It is shown that the number of individuals to be used for estimating average heterozygosity can be very small if a large number of loci are studied and the average heter homozygosity is low.
Related Papers (5)
Mathematical model for studying genetic variation in terms of restriction endonucleases
Masatoshi Nei,Wen-Hsiung Li +1 more