Journal ArticleDOI
Alginate as immobilization matrix and stabilizing agent in a two-phase liquid system: application in lipase-catalysed reactions.
TLDR
By using the hydrophilic alginate gel as an immobilization matrix in combination with a mobile hydrophobic phase, a two-phase liquid system was achieved with definite advantages for a continuous, enzyme-catalysed process.About:
This article is published in Enzyme and Microbial Technology.The article was published on 1992-01-01. It has received 82 citations till now. The article focuses on the topics: Calcium alginate & Immobilized enzyme.read more
Citations
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Journal ArticleDOI
Main properties and current applications of some polysaccharides as biomaterials
TL;DR: The main polysaccharides currently used in the biomedical and pharmaceutical domains are chitin and its derivative chitosan, hyaluronan, and alginates.
Journal ArticleDOI
Customizing lipases for biocatalysis: a survey of chemical, physical and molecular biological approaches
TL;DR: The present review will survey the different procedures that have been developed to enhance the properties of lipases by physical modifications of the biocatalysts by adsorption on a carrier material, entrapment or microencapsulation.
Journal ArticleDOI
Bioreactors with immobilized lipases: state of the art.
TL;DR: This review attempts to provide an updated compilation of studies reported in the literature pertaining to reactors containing lipases in immobilized forms, in a way that helps the reader direct a bibliographic search and develop an integrated perspective of the subject.
Journal ArticleDOI
Whole-cell biocatalysis in organic media
TL;DR: Correlations between the cellular toxicity or the extractive capacities of different solvents and some of their physical properties have been proposed in order to minimize preliminary, solvent-selection experimental work but also to help in the understanding of the molecular mechanisms of toxicity and extraction.
Journal ArticleDOI
Immobilization of lipase using hydrophilic polymers in the form of hydrogel beads.
Seema S Betigeri,Steven H. Neau +1 more
TL;DR: It can be concluded that chitosan is a polymer worthy of pursuit to immobilize lipase, after evaluating enzyme loading, leaching, and activity in beads using various polymers from Candida rugosa.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Alginate as immobilization matrix for cells
Olav Smidsrød,Gudmund Skjåk-Bræk +1 more
TL;DR: This review evaluates the potential of entrapment of cells within spheres of Ca2+ alginate gels on the basis of the current knowledge of structural and functional relationships in alginATE gels.
Journal ArticleDOI
Alginate as immobilization material: I. Correlation between chemical and physical properties of alginate gel beads
TL;DR: Beads with the highest mechanical strength, lowest shrinkage, best stability towards monovalent cations, and highest porosity were made from alginate with a content of L‐guluronic acid higher than 70% and an average length of the G‐blocks higher than 15.4 × 105.
Journal ArticleDOI
Enzymatic catalysis in anhydrous organic solvents
TL;DR: Not only do enzymes work vigorously in anhydrous organic media, but in this unnatural milieu they acquire remarkable properties such as greatly enhanced stability, radically altered substrate and enantiomeric specificities, molecular memory, and the ability to catalyse unusual reactions.
Journal ArticleDOI
Immobilized Enzymes and Cells as Practical Catalysts
TL;DR: Applications of immobilized enzymes and cells in the chemical, pharmaceutical, and food industries, in clinical and chemical analyses, and in medicine, as well as probable future trends in enzyme technology are discussed.
Related Papers (5)
Immobilization of lipase using hydrophilic polymers in the form of hydrogel beads.
Seema S Betigeri,Steven H. Neau +1 more