All intermediates of the arsenate reductase mechanism, including an intramolecular dynamic disulfide cascade.
Joris Messens,José C. Martins,Karolien Van Belle,Elke Brosens,Aline Desmyter,Marjan De Gieter,Jean-Michel Wieruszeski,Rudolph Willem,Lode Wyns,Ingrid Zegers +9 more
TLDR
The mechanism of pI258 arsenate reductase (ArsC) catalyzed arsenate reduction, involving its P-loop structural motif and three redox active cysteines, has been unraveled and Steady-state kinetics of ArsC mutants gives a view of the crucial residues for catalysis.Abstract:
The mechanism of pI258 arsenate reductase (ArsC) catalyzed arsenate reduction, involving its P-loop structural motif and three redox active cysteines, has been unraveled. All essential intermediates are visualized with x-ray crystallography, and NMR is used to map dynamic regions in a key disulfide intermediate. Steady-state kinetics of ArsC mutants gives a view of the crucial residues for catalysis. ArsC combines a phosphatase-like nucleophilic displacement reaction with a unique intramolecular disulfide bond cascade. Within this cascade, the formation of a disulfide bond triggers a reversible “conformational switch” that transfers the oxidative equivalents to the surface of the protein, while releasing the reduced substrate.read more
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Bacterial silver resistance: molecular biology and uses and misuses of silver compounds
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The changing faces of glutathione, a cellular protagonist
TL;DR: The significance of GSH as a major factor in regulation of cell life, proliferation, and death, should be regarded as the integrated result of all these roles it can play.
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Microbial arsenic: from geocycles to genes and enzymes.
TL;DR: The DNA sequencing and protein crystal structures have established the convergent evolution of three classes of arsenate reductases, which involve three cysteine thiols and S-As bond intermediates, so convergence evolution to similar mechanisms has taken place.
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Arsenic binding to proteins.
TL;DR: Although the adverse health effects arising from exposure to arsenic have been well-recognized, the mechanism(s) of action responsible for the diverse range of health effects are complicated and poorly understood.
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Genes and Enzymes Involved in Bacterial Oxidation and Reduction of Inorganic Arsenic
Simon Silver,Le T. Phung +1 more
TL;DR: The human use of toxic heavy metals is here to stay, and medical, agricultural, and industrial uses of arsenic present major challenges.
References
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