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Open AccessJournal ArticleDOI

Am E. coli gene product required for λ site-specific recombination

Harvey I. Miller, +1 more
- 01 Jul 1980 - 
- Vol. 20, Iss: 3, pp 711-719
TLDR
In this paper, the authors report characteristics of E. coli mutations of the himA allele, selected for their inability to support the site-specific recombination reaction involved in the formation of lysogens by bacteriophage lambda.
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This article is published in Cell.The article was published on 1980-07-01 and is currently open access. It has received 163 citations till now. The article focuses on the topics: Site-specific recombination.

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Citations
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Journal ArticleDOI

Global gene expression profiling in Escherichia coli K12. The effects of integration host factor.

TL;DR: The results demonstrate that small differences in gene expression levels can be accurately measured and that the significance of differential gene expression measurements cannot be assessed simply by the magnitude of the fold difference.
Journal ArticleDOI

E. coli integration host factor binds to specific sites in DNA

TL;DR: From comparison of the sequences protected by IHF, it is suggested that the critical determinant in specific IHF-DNA interaction is contained in the sequence T.A.PuTT...PyAACtA.PyAA...Pu TTGaT.
Journal ArticleDOI

Purification and properties of the Escherichia coli protein factor required for lambda integrative recombination.

TL;DR: A purified preparation of the Escherichia coli integration host factor displays two polypeptides of apparent molecular weight 11,000 and 9,500 when analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.
References
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Book

Experiments in molecular genetics

TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI

Integration-negative mutants of bacteriophage lambda.

TL;DR: The recombinational behavior of int mutants in wild-type and recombination-deficient bacteria suggests that the int + gene determines a site-specific function involved in normal prophage integration and detachment.
Journal ArticleDOI

Prophage lambda at unusual chromosomal locations: I. Location of the secondary attachment sites and the properties of the lysogens

TL;DR: The integration, frequency of phage λ into a mutant host deleted for the normal prophage insertion site is reduced about 200-fold relative to integration into wild-type Escherichia coli.
Journal ArticleDOI

Transposition of R factor genes to bacteriophage lambda

TL;DR: The properties of the lambdakan phage suggest that R factors contain systems capable of mediating genetic exchange in the absence of extensive DNA homology, and it is suggested that such systems of exchange may have played important roles in R factor evolution.