An Aluminum-Activated Citrate Transporter in Barley
Jun Furukawa,Naoki Yamaji,Hua Wang,Namiki Mitani,Yoshiko Murata,Kazuhiro Sato,Maki Katsuhara,Kazuyoshi Takeda,Jian Feng Ma +8 more
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TLDR
It is demonstrated that HvAACT1 is an Al-activated citrate transporter responsible for Al resistance in barley.Abstract:
Soluble ionic aluminum (Al) inhibits root growth and reduces crop production on acid soils Al-resistant cultivars of barley (Hordeum vulgare L) detoxify Al by secreting citrate from the roots, but the responsible gene has not been identified yet Here, we identified a gene (HvAACT1) responsible for the Al-activated citrate secretion by fine mapping combined with microarray analysis, using an Al-resistant cultivar, Murasakimochi, and an Al-sensitive cultivar, Morex This gene belongs to the multidrug and toxic compound extrusion (MATE) family and was constitutively expressed mainly in the roots of the Al-resistant barley cultivar Heterologous expression of HvAACT1 in Xenopus oocytes showed efflux activity for (14)C-labeled citrate, but not for malate Two-electrode voltage clamp analysis also showed transport activity of citrate in the HvAACT1-expressing oocytes in the presence of Al Overexpression of this gene in tobacco enhanced citrate secretion and Al resistance compared with the wild-type plants Transiently expressed green fluorescent protein-tagged HvAACT1 was localized at the plasma membrane of the onion epidermal cells, and immunostaining showed that HvAACT1 was localized in the epidermal cells of the barley root tips A good correlation was found between the expression of HvAACT1 and citrate secretion in 10 barley cultivars differing in Al resistance Taken together, our results demonstrate that HvAACT1 is an Al-activated citrate transporter responsible for Al resistance in barleyread more
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References
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A silicon transporter in rice
Jian Feng Ma,Kazunori Tamai,Naoki Yamaji,Namiki Mitani,Saeko Konishi,Maki Katsuhara,Masaji Ishiguro,Yoshiko Murata,Masahiro Yano +8 more
TL;DR: The identification of a silicon transporter provides both an insight into the silicon uptake system in plants, and a new strategy for producing crops with high resistance to multiple stresses by genetic modification of the root's silicon uptake capacity.
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Function and mechanism of organic anion exudation from plant roots
TL;DR: The benefits that plants derive from the presence of organic anions in the rhizosphere are described and the potential for biotechnology to increase organic anion exudation is highlighted.
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Global extent, development and economic impact of acid soils
H. R. von Uexküll,E. W. Mutert +1 more
TL;DR: Acid soils occupy approximately 30% or 3950 m ha of the world's ice free land area and occur mainly in two global belts where they have developed under udic or ustic moisture regimes as mentioned in this paper.
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Aluminium tolerance in plants and the complexing role of organic acids
TL;DR: A range of plant species has evolved mechanisms that enable them to grow on acid soils where toxic concentrations of Al(3+) can limit plant growth, and organic acids play a central role in these aluminium tolerance mechanisms.
Journal ArticleDOI
Aluminum Tolerance in Wheat (Triticum aestivum L.) (II. Aluminum-Stimulated Excretion of Malic Acid from Root Apices)
TL;DR: There was a consistent correlation of Al tolerance with high rates of malic acid excretion stimulated by Al in a population of seedlings segregating for Al tolerance, consistent with the hypothesis that the Alt1 locus in wheat encodes an Al tolerance mechanism based on Al-stimulated excretion ofmalic acid.
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