Journal ArticleDOI
Analysis of myosin heavy chains at the protein level in horse skeletal muscle.
TLDR
These integrated methods for the analysis of MHCs at the protein level demonstrate that equine skeletal muscle does not express the MHC-IIB, so type II fibres have been misclassified in numerous previous studies based upon the very traditional mATPase histochemistry.Abstract:
Combined methodologies of enzyme-linked immunosorbent assay (ELISA), sodium dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting, traditional myofibrillar ATPase (mATPase) histochemistry and immunocytochemistry of whole biopsied samples were used to study myosin heavy chain (MHC) isoforms in the equine gluteus medius muscle. The ELISA technique allowed the quantification of the three MHC isoforms known to be present in different horse muscles: slow (MHC-I) and two fast (termed MHC-IIA and MCH-IIX). The SDS-PAGE method resolved MHCs in three bands: MHC-I, MHC-IIX and MHC-IIA from the fastest to the slowest migrating band and a quantification by densitometry for each MHC isoform was also possible. The identity of these three MHCs was confirmed by immunoblots with specific monoclonal antibodies. Five fibre types were defined immunohistochemically according to their MHC content: I, I + IIA, IIA, the hybrid IIAX and IIX. When quantitative data obtained with the four different methodologies were combined and compared, they were consistent and, when considered together, showed significant correlation. Nevertheless, the percentage of MHC-IIA histochemically derived was underestimated, while that of MHC-IIX was overestimated in comparison with the immunocytochemical determination of these MHC isoforms. The percentage of MHC-I obtained by ELISA technique was underestimated. In short, these integrated methods for the analysis of MHCs at the protein level demonstrate that equine skeletal muscle does not express the MHC-IIB, so type II fibres have been misclassified in numerous previous studies based upon the vary traditional mATPase histochemistry. They also offer new prospects for muscle fibre typing in equine experimental studies and veterinary medicine.read more
Citations
More filters
Journal ArticleDOI
Fiber types in mammalian skeletal muscles.
TL;DR: Mammalian skeletal muscle comprises different fiber types, whose identity is first established during embryonic development by intrinsic myogenic control mechanisms and is later modulated by neural and hormonal factors.
Journal ArticleDOI
Human skeletal muscle fibres: molecular and functional diversity
TL;DR: The present review will describe the mechanisms through which molecular diversity is generated and how fibre types can be identified on the basis of structural and functional characteristics and discuss the advantage that fibre diversity can offer in optimizing muscle contractile performance.
Journal ArticleDOI
A second look into fibre typing--relation to meat quality.
TL;DR: This review describes the involvements of Ca2+-dependent mechanisms, and the energy state of the myofibres in the control of contractile and metabolic properties, and proposes some genetic and environmental factors as possible tools to control meat quality trough the modulation of fibre type characteristics.
Journal ArticleDOI
New insights into muscle fiber types in the pig
TL;DR: According to the type grouping distribution of myofibers encountered in pig muscle, MyHC isoform expression followed the rank order of I→Iia→Iix→IIb from the center to the periphery of the islets, concomitantly with a decrease in oxidative metabolism and an increase in fiber size.
Journal ArticleDOI
Laryngeal muscle fibre types
TL;DR: The concepts of allotype and phylogenetic plasticity help to explain differences in fibre type between limb and laryngeal muscles and between homologous larynGEal muscles in different species.
References
More filters
Journal ArticleDOI
Muscle Fiber Types: How Many and What Kind?
TL;DR: The purpose of the present communication is to point out some of the problems involved in the classification of fibers and to add new information of value in the analysis of human biopsy material.
Book
Muscle Biopsy: A Practical Approach
TL;DR: The procedure of muscle biopsy and definition of pathological changes seen in muscle biopsies are described, as well as some of the commonly used terms.
Journal ArticleDOI
Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
Stefano Schiaffino,Luisa Gorza,Saverio Sartore,Leopoldo Saggin,Simonetta Ausoni,M. Vianello,Kristian Gundersen,Terje Lømo +7 more
TL;DR: The existence of the 2X-MHC isoform was confirmed by immunoblotting analysis using muscles containing 2X fibres as a major component, such as the normal and hyperthyroid diaphragm, and the soleus muscle after high frequency chronic stimulation.
Journal ArticleDOI
Electrophoretic separation of rat skeletal muscle myosin heavy-chain isoforms
Robert J. Talmadge,Roland R. Roy +1 more
TL;DR: The percent composition of the four adult MHCs in rat soleus, medial gastrocnemius, diaphragm, and levator ani muscles by use of this procedure and Coomasie Blue staining is similar to that previously reported.
Book ChapterDOI
Mammalian skeletal muscle fiber type transitions
Dirk Pette,Robert S. Staron +1 more
TL;DR: A simple transition scheme has emerged from the multitude of data collected on fiber type conversions under a variety of conditions, and it is now clear that fiber type transitions do not proceed in immediate jumps from one extreme to the other, but occur in a graded and orderly sequential manner.
Related Papers (5)
Molecular diversity of myofibrillar proteins: gene regulation and functional significance
Fibre composition, enzyme activity and concentrations of metabolites and electrolytes in muscles of standardbred horses.
Arne Lindholm,Karin Piehl +1 more