Anti-oxidant, anti-apoptotic, anti-hypoxic and anti-inflammatory conditions induced by PTY-2 against STZ-induced stress in islets.
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The results showed that PTY-2 favorably changed all the expressions via anti-oxidant, anti-apoptotic,Anti-hypoxic and anti-inflammatory pathways, making itself as a protective agent against streptozotocin induced islet stress in rats.Abstract:
The earlier assessment of Pueraria tuberosa (PT) has shown anti-diabetic effects through enhancing incretin action and DPP-IV (Dipeptidyl peptidase-IV) inhibition. The aim of this work was to further explore the protective role of aqueous extract of Pueraria tuberosa tuber (PTY-2) against streptozotocin (STZ) induced islet stress in rats. Diabetes was induced by STZ (65 mg/kg body weight) in charles foster male rats. After 60 days of STZ administration, animals with blood glucose levels > 200 g/dL were considered as diabetic. All the rats were later divided into three groups: Group-1 (STZ untreated normal rats), Group-2 (Diabetic control), and Group-3 (PTY-2 [50 mg/100 g bw treatment for next 10 days to diabetic rats). The rats were then sacrificed after the 10th day of treatment accordingly. STZ treatment led to an increase in expression of Matrix metalloproteinases-9 (MMP-9), Tumour necrosis factor-α (Tnf-α), Hypoxia inducible factor-α (HIF-1α), Vascular endothelial growth factor (VEGF), Interleukin-6 (IL-6), Protein kinase C-e (PKC-e), Nuclear factor kappa-light-chain-enhancer of activated B-cells (NFkB), and Caspase-3. Reverse Transcriptase-PCR (RT-PCR), Immunohistochemistry and Western-Blot analysis showed an increase in the expressions of Superoxide Dismutase (SOD) and Nephrin, and a decrease in the expressions of NFkB, PKC-e, TNF-α, MMP-9, HIF-1α, VEGF, Caspase-3 and IL-6 after 10 days of PTY-2 treatment. The results showed that PTY-2 favorably changed all the expressions via anti-oxidant, anti-apoptotic, anti-hypoxic and anti-inflammatory pathways, making itself as a protective agent against STZ induced islet stress. Further evaluation of PTY-2 might be helpful in establishing its role in the management of diabetes mellitus.read more
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Journal ArticleDOI
Pueraria tuberosa: A Review on Traditional Uses, Pharmacology, and Phytochemistry
Ram Bharti,Ram Bharti,Bhupinder Singh Chopra,Bhupinder Singh Chopra,Sachin Raut,Sachin Raut,Neeraj Khatri,Neeraj Khatri +7 more
TL;DR: Pueraria tuberosa (Roxb. ex Willd.) DC. as mentioned in this paper is a perennial herb distributed throughout India and other Asian countries, and its extracts have been reported for nutritional and medicinal properties in Ayurveda as well as in Chinese traditional practices.
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An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0.
Jinli Hou,Jinli Hou,Xiang Zhou,Ping Wang,Chunhui Zhao,Yuewen Qin,Feng Liu,Liping Yu,Haiyu Xu +8 more
TL;DR: Wang et al. as mentioned in this paper found that purslane seed (MCXZ) treatment resulted in hypoglycemic activity, lowering the fasting blood glucose and glycated hemoglobin levels in streptozotocin-induced diabetic mice.
Journal ArticleDOI
Structural characterization, anti-inflammatory and glycosidase inhibitory activities of two new polysaccharides from the root of Pueraria lobata
Jiale Cai,Xiaoting Zhong,Jiayin Liang,Can Xu,Huanzheng Yu,Minghua Xian,Chunyan Yan,Shumei Wang +7 more
TL;DR: In this paper, two new polysaccharides (PLB-1-1 and PLB- 1-2) were isolated and purified from the root of Pueraria lobata with molecular weights of 9.1 × 103 Da and 3.8 × 103 da, respectively, and evaluated by monosaccharide composition, GC-MS and NMR spectroscopy.
Posted ContentDOI
Histopathological study of different organs of charles foster strain rat under the exposure of Pueraria tuberosa.
TL;DR: No adverse alterations in cardiac fibers of the heart, size and shapes in crypts and villi of intestine, seminiferous tubules and spermatozoa count in testis, three zones of adrenal gland, and spleen were seen in all treated groups of PTWE.
Journal ArticleDOI
Pueraria tuberosa as Dipeptidyl-Peptidase-IV Inhibitor Prevents Streptozotocin-Induced Intestinal Stress
TL;DR: The protective action of PTY-2 against STZ mediated intestinal damage is demonstrated and may be taken as a herbal remedy for diabetes-induced intestinal damages.
References
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Journal ArticleDOI
Pancreas-specific RelA/p65 truncation increases susceptibility of acini to inflammation-associated cell death following cerulein pancreatitis
Hana Algül,Matthias Treiber,Marina Lesina,Hassan Nakhai,Dieter Saur,Fabian Geisler,Alexander Pfeifer,Stephan Paxian,Roland M. Schmid +8 more
TL;DR: In vivo evidence for RelA/p65 protection of acinar cell death via upregulation of PAP1 is provided and multidimensional roles of NF-kappaB/Rel in different cells and contexts during inflammation are suggested.
Journal ArticleDOI
Caspase-3-Dependent β-Cell Apoptosis in the Initiation of Autoimmune Diabetes Mellitus
Nicole Liadis,Kiichi Murakami,Mohamed Eweida,Alisha R. Elford,Laura Sheu,Herbert Y. Gaisano,Razqallah Hakem,Pamela S. Ohashi,Minna Woo +8 more
TL;DR: It is shown that Caspase-3 knockout (Casp3− /−) mice were protected from developing diabetes in a multiple-low-dose streptozotocin autoimmune diabetes model and demonstrate that caspase3-mediated β-cell apoptosis is a requisite step for T-cell priming, a key initiating event in type 1 diabetes.
Journal ArticleDOI
Apoptotic signal transduction pathways in diabetes
TL;DR: It is hypothesized that immunological stimuli in type 1 diabetes and metabolic/inflammatory signals in type 2 diabetes converge on common signalling pathways leading to beta-cell failure and destruction in these two diseases.
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Prognostic significance of HIF-1 alpha overexpression in human pancreatic cancer.
Takamune Shibaji,Mitsuo Nagao,Naoya Ikeda,Hiromichi Kanehiro,Michiyoshi Hisanaga,Saiho Ko,Akihisa Fukumoto,Yoshiyuki Nakajima +7 more
TL;DR: Assessment of HIF-1 alpha expression might be useful for predicting the prognosis of pancreatic cancer patients, which contributes to angiogenesis and progression.
Journal ArticleDOI
Association of TNF-α with insulin resistance in type 2 diabetes mellitus.
TL;DR: The results suggest the possible role of TNF-α in the pathogenesis of type-2 diabetes mellitus and the importance of reducing obesity to prevent elevated levels of the cytokine and related complications.