Anti-oxidant, anti-apoptotic, anti-hypoxic and anti-inflammatory conditions induced by PTY-2 against STZ-induced stress in islets.
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The results showed that PTY-2 favorably changed all the expressions via anti-oxidant, anti-apoptotic,Anti-hypoxic and anti-inflammatory pathways, making itself as a protective agent against streptozotocin induced islet stress in rats.Abstract:
The earlier assessment of Pueraria tuberosa (PT) has shown anti-diabetic effects through enhancing incretin action and DPP-IV (Dipeptidyl peptidase-IV) inhibition. The aim of this work was to further explore the protective role of aqueous extract of Pueraria tuberosa tuber (PTY-2) against streptozotocin (STZ) induced islet stress in rats. Diabetes was induced by STZ (65 mg/kg body weight) in charles foster male rats. After 60 days of STZ administration, animals with blood glucose levels > 200 g/dL were considered as diabetic. All the rats were later divided into three groups: Group-1 (STZ untreated normal rats), Group-2 (Diabetic control), and Group-3 (PTY-2 [50 mg/100 g bw treatment for next 10 days to diabetic rats). The rats were then sacrificed after the 10th day of treatment accordingly. STZ treatment led to an increase in expression of Matrix metalloproteinases-9 (MMP-9), Tumour necrosis factor-α (Tnf-α), Hypoxia inducible factor-α (HIF-1α), Vascular endothelial growth factor (VEGF), Interleukin-6 (IL-6), Protein kinase C-e (PKC-e), Nuclear factor kappa-light-chain-enhancer of activated B-cells (NFkB), and Caspase-3. Reverse Transcriptase-PCR (RT-PCR), Immunohistochemistry and Western-Blot analysis showed an increase in the expressions of Superoxide Dismutase (SOD) and Nephrin, and a decrease in the expressions of NFkB, PKC-e, TNF-α, MMP-9, HIF-1α, VEGF, Caspase-3 and IL-6 after 10 days of PTY-2 treatment. The results showed that PTY-2 favorably changed all the expressions via anti-oxidant, anti-apoptotic, anti-hypoxic and anti-inflammatory pathways, making itself as a protective agent against STZ induced islet stress. Further evaluation of PTY-2 might be helpful in establishing its role in the management of diabetes mellitus.read more
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Pueraria tuberosa: A Review on Traditional Uses, Pharmacology, and Phytochemistry
Ram Bharti,Ram Bharti,Bhupinder Singh Chopra,Bhupinder Singh Chopra,Sachin Raut,Sachin Raut,Neeraj Khatri,Neeraj Khatri +7 more
TL;DR: Pueraria tuberosa (Roxb. ex Willd.) DC. as mentioned in this paper is a perennial herb distributed throughout India and other Asian countries, and its extracts have been reported for nutritional and medicinal properties in Ayurveda as well as in Chinese traditional practices.
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An Integrative Pharmacology-Based Approach for Evaluating the Potential Effects of Purslane Seed in Diabetes Mellitus Treatment Using UHPLC-LTQ-Orbitrap and TCMIP V2.0.
Jinli Hou,Jinli Hou,Xiang Zhou,Ping Wang,Chunhui Zhao,Yuewen Qin,Feng Liu,Liping Yu,Haiyu Xu +8 more
TL;DR: Wang et al. as mentioned in this paper found that purslane seed (MCXZ) treatment resulted in hypoglycemic activity, lowering the fasting blood glucose and glycated hemoglobin levels in streptozotocin-induced diabetic mice.
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Structural characterization, anti-inflammatory and glycosidase inhibitory activities of two new polysaccharides from the root of Pueraria lobata
Jiale Cai,Xiaoting Zhong,Jiayin Liang,Can Xu,Huanzheng Yu,Minghua Xian,Chunyan Yan,Shumei Wang +7 more
TL;DR: In this paper, two new polysaccharides (PLB-1-1 and PLB- 1-2) were isolated and purified from the root of Pueraria lobata with molecular weights of 9.1 × 103 Da and 3.8 × 103 da, respectively, and evaluated by monosaccharide composition, GC-MS and NMR spectroscopy.
Posted ContentDOI
Histopathological study of different organs of charles foster strain rat under the exposure of Pueraria tuberosa.
TL;DR: No adverse alterations in cardiac fibers of the heart, size and shapes in crypts and villi of intestine, seminiferous tubules and spermatozoa count in testis, three zones of adrenal gland, and spleen were seen in all treated groups of PTWE.
Journal ArticleDOI
Pueraria tuberosa as Dipeptidyl-Peptidase-IV Inhibitor Prevents Streptozotocin-Induced Intestinal Stress
TL;DR: The protective action of PTY-2 against STZ mediated intestinal damage is demonstrated and may be taken as a herbal remedy for diabetes-induced intestinal damages.
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