Antigenic diversity and similarities detected in avian paramyxovirus type 1 (Newcastle disease virus) isolates using monoclonal antibodies.
D. J. Alexander,Ruth J. Manvell,J. P. Lowings,K. M. Frost,M. S. Collins,P.H. Russell,J. E. Smith +6 more
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Cluster analysis of the mAb binding patterns did not produce concise, discrete groupings, but did emphasise some relationships between virus properties and antigenicity, especially for viruses causing discrete epizootics.Abstract:
Summary Newcastle disease (ND) virus (APMV‐1) isolates submitted to the International Reference Laboratory for ND were characterised antigenically by their ability to cause binding of mouse monoclonal antibodies (mAbs) to cell cultures infected with the isolate. Since the availability of the mAbs 1526 viruses have been examined using a panel of nine mAbs and 818 with an extended panel of 26 mAbs. Using the nine mAb panel a total of 14 different patterns was seen and viruses grouped by the same pattern showed relationships with each other which were either biological, temporal or geographical or more than one of these. There was a marked tendency of viruses placed in the same group to show similar virulence for chickens. Extension of the panel to 26 mAbs produced 39 distinct patterns, although some of these were seen with only a single virus. Again, viruses inducing similar binding patterns shared similar properties and some binding patterns were specific for viruses causing discrete epizootics. Cluster an...read more
Citations
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Journal ArticleDOI
Mapping the Antigenic and Genetic Evolution of Influenza Virus
Derek J. Smith,Derek J. Smith,Alan Lapedes,Jan C. de Jong,Theo M. Bestebroer,Guus F. Rimmelzwaan,Albert D. M. E. Osterhaus,Ron A. M. Fouchier +7 more
TL;DR: The antigenic evolution of influenza A (H3N2) virus was quantified and visualized from its introduction into humans in 1968 to 2003 and offers a route to predicting the relative success of emerging strains.
Journal ArticleDOI
Newcastle disease and other avian paramyxoviruses.
TL;DR: Newcastle disease (ND), caused by avian paramyxovirus serotype 1 (APMV-1) viruses, is included in List A of the Office International des Epizooties as mentioned in this paper.
Journal ArticleDOI
Third genome size category of avian paramyxovirus serotype 1 (Newcastle disease virus) and evolutionary implications
TL;DR: The goal of the study was to establish if there was a relationship between molecular patterns and virus evolution, and the complete genome sequence of two distinct apathogenic Newcastle disease virus (NDV) strains was determined and a third genome size category, containing 15,198 nucleotides, was recognized.
Journal ArticleDOI
Newcastle disease: Evolution of genotypes and the related diagnostic challenges
TL;DR: The molecular epidemiology and recent diagnostic problems related to viral evolution of NDV are reviewed and it is explained why a new system, based on objective criteria, is needed to categorize genotypes.
Journal ArticleDOI
Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1).
E W Aldous,D J Alexander +1 more
TL;DR: Panels of monoclonal antibodies were a major advance for the characterization of NDV isolates, although confirmation of virulence for poultry still required in vivo testing and molecular-based techniques become easier and more reliable.
References
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Book ChapterDOI
Newcastle Disease in Free-Living and Pet Birds
TL;DR: All of the approximately 8,000 species of birds seem to be susceptible to infection with Newcastle disease viruses (NDVs), so efforts are needed to protect birds from these viruses.
Journal ArticleDOI
Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity.
TL;DR: Despite the extremely small portion of the genome studied there were several areas which appeared characteristic for 34/90 and the three group H viruses of low virulence, which suggests that they may have arisen from the same gene pool.
Journal ArticleDOI
Identification and grouping of Newcastle disease virus strains by restriction site analysis of a region from the F gene
TL;DR: It is concluded that restriction site analysis of F gene PCR amplicons is a relatively fast, simple and reliable method for the differentiation and identification of NDV strains.
Journal ArticleDOI
Antigenic variation of Newcastle disease virus strains detected by monoclonal antibodies.
P. H. Russell,D. J. Alexander +1 more
TL;DR: Forty Newcastle disease virus strains and isolates could be placed in eight distinct antigenic groups on their ability to induce binding of nine mouse monoclonal antibodies to infected MDBK cells as assessed by an indirect immuno-peroxidase test.