Journal ArticleDOI
"Arabidobrassica": A novel plant obtained by protoplast fusion.
Yury Yu. Gleba,F. Hoffmann +1 more
TLDR
Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated, representing the first case of intergeneric-intertribal hybridization of flowering plants.Abstract:
Using somatic hybrid cell lines Arabidopsis thaliana+Brassica campestris, obtained by cloning individual protoplast-fusion products as starting material, shoots and flowering plants have been regenerated. Cytological, biochemical, and morphological analyses indicate that genetic material of both species is present in the resultant plants. Shoots and plants obtained from different lines and different regeneration events differed morphologically and genetically. Most regenerants show morphological abnormalities and unusual organizational patterns. Flowering forms have so far been sterile. "Asymmetric" hybrids (i.e., hybrids bearing most genetic material of one of the parent species and only few chromosomes of the other) were more regular in morphology. The results represent the first case of intergeneric-intertribal hybridization of flowering plants.read more
Citations
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High efficiency transformation ofBrassica napus usingAgrobacterium vectors.
TL;DR: The frequency of transformation and yield of transgenic plants was higher than in previously reported methods with this species and Southern blotting revealed that integration of the T-DNA frequently occurred in multiple copies and at multiple loci in the genome.
Journal ArticleDOI
Cytogenetics of plant cell and tissue cultures and their regenerates
Francesco D'Amato,M. W. Bayliss +1 more
TL;DR: The cytogenetics of plant cell and tissue cultures and their regenerates will be discussed, and nuclear processes at and during callus induction will be followed.
Patent
Plasmids for transforming plant cells
TL;DR: In this article, a co-integrate Ti plasmid is inserted into a suitable microorganism, such as A. tumefaciens, which contains a Ti-plasmid.
Journal ArticleDOI
Intergeneric somatic hybridization and its application to crop genetic improvement
TL;DR: Somatic cell fusion has potential applications for crop genetic improvement by overcoming sexual incompatibility or reproductive barriers, and by realizing novel combinations of nuclear and/or cytoplasmic genomes.
Journal ArticleDOI
The mutants of Arabidopsis
Mark Estelle,Chris Somerville +1 more
TL;DR: The small crucifer Arabidopsis thaliana (L.) Heynh, has many advantages as a model system for plant molecular genetics and should be facilitated by a relatively small and simple genome.
References
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Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Somatic hybrid plants of potato and tomato regenerated from fused protoplasts
TL;DR: In the absence of a selection system against the potato parent, the analysis of ribulose bisphosphate carboxylase provides a convenient marker to demonstrate the hybrid nature of the plants.
Book
The biology and chemistry of the Cruciferae
TL;DR: The biology and chemistry of the Cruciferae is studied in detail in order to establish a clear picture of the phylum and its phytochemical secrets.
Journal ArticleDOI
Hybrid cell lines Arabidopsis thaliana + Brassica campestris : No evidence for specific chromosome elimination
Yury Yu. Gleba,F. Hoffmann +1 more
TL;DR: The data obtained are interpreted as evidence for retention and functional activity in hybrid cells of specific chromosomes from each species for at least 7 months of culture.
Journal ArticleDOI
Restoration of morphogenic potential in Nicotiana by somatic hybridisation
TL;DR: Shot formation on inductive medium was restored, indicating that the inducibility factor had not been altered in KR103, and genetic complementation and induced regulatory changes due to genome interaction are discussed as possible explanations for the restoration of morphogenic potential.
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