Association of lactoferrin with specific granules in rabbit heterophil leukocytes
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Leukocyte lactoferrin was found to be exclusively localized in the specific (secondary) granules, which have been resolved from other subcellular components by zonal differential centrifugation and by isopycnic equilibration.Abstract:
Lactoferrin has been identified in rabbit heterophil leukocytes on the basis of its immunological reactivity, electrophoretic mobility, acid-resistant iron-binding properties, and spectral characteristics. Leukocyte lactoferrin was found to be exclusively localized in the specific (secondary) granules, which have been resolved from other subcellular components by zonal differential centrifugation and by isopycnic equilibration.read more
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References
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Resolution of granules from rabbit heterophil leukocytes into distinct populations by zonal sedimentation
TL;DR: Postnuclear supernates from homogenates of essentially pure rabbit heterophil leukocytes were fractionated by means of zonal differential centrifugation through a discontinuous sucrose gradient at various speeds and three distinct groups of granules were characterized biochemically and morphologically.
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Phagocytin: a bactericidal substance from polymorphonuclear leucocytes.
TL;DR: Although phagocytin is reasonably stable at temperatures of 65°C.
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Electron microscope studies on the degranulation of rabbit peritoneal leukocytes during phagocytosis.
TL;DR: It has been proposed that the release of material to the outside of the cell or the discharge of material into intracellular vacuoles, events involving membrane fusion, be termed "exoplasmosis."
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Preparation and properties of lactosiderophilin (lactotransferrin) of human milk
TL;DR: In this article, the authors describe a method of isolating and purifying from human milk a mucoid that has, like plasma siderophilin, the ability to bind iron reversibly.