Association of lactoferrin with specific granules in rabbit heterophil leukocytes
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Leukocyte lactoferrin was found to be exclusively localized in the specific (secondary) granules, which have been resolved from other subcellular components by zonal differential centrifugation and by isopycnic equilibration.Abstract:
Lactoferrin has been identified in rabbit heterophil leukocytes on the basis of its immunological reactivity, electrophoretic mobility, acid-resistant iron-binding properties, and spectral characteristics. Leukocyte lactoferrin was found to be exclusively localized in the specific (secondary) granules, which have been resolved from other subcellular components by zonal differential centrifugation and by isopycnic equilibration.read more
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Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats.
Guang Yang,Christopher Qian,Chao Zhang,Yong Bao,Meng-Yue Liu,Fei Jiang,Wei Li,Yong Liu,Ya Ke,Zhong-Ming Qian,Zhong-Ming Qian +10 more
TL;DR: It is demonstrated that hepcidin could significantly suppress the ICH-induced increase in iron and ferritin in brain tissues and CSF by inhibiting expression of iron transport proteins, increase neuronal survival by attenuating I CH-induced apoptosis, reactive oxygen species, neurodegeneration and brain edema, as well as effectively improve ICH -induced behavioral and cognitive deficit in rats.
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Ovine lactoferrin: isolation from colostrum and characterization.
TL;DR: Highly purified lactoferrin was isolated from ovine colostrum by sequential purification on CM-Sephadex C-50 and Blue-Sepharose, with overall yield of 55%.
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Exocytosis induced in neutrophils by chemotactic agents and other stimuli
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Immunochemical quantification of in vitro neutrophilic granulocyte differentiation
TL;DR: To the knowledge of this work, this is the first demonstration of a quantitative relationship at both the biochemical and cellular levels between CSA and a specific marker of neutrophilic granulocyte differentiation.
References
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Immunochemical quantitation of antigens by single radial immunodiffusion
TL;DR: By standardizing the technical conditions of the experiment it is possible to use this principle for the immunochemical determination of antigens, and the lower limit of the method was found to correspond to 0·0025 μg of antigen, and to an antigen concentrations of 1·25 μg per ml.
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The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions.
Federico Leighton,Brian Poole,Henri Beaufay,Pierre Baudhuin,John W. Coffey,Stanley Fowler,Christian de Duve +6 more
TL;DR: Improved, largely automated methods are described for the purification and analysis of peroxisomes, lysosomes, and mitochondria from the livers of rats injected with Triton WR-1339, making more conclusive and precise previous estimates of the biochemical and morphological properties of the three groups of cytoplasmic particles.
Journal ArticleDOI
Lactoferrin, an iron-binbing protein ni neutrophilic leukocytes
TL;DR: Immunohistochemical data indicate that lactoferrin first appears in myeloid cells at the stage of the promyelocyte, and the identification of this protein in leukocyte extracts was based upon a comparison of its electrophoretic, antigenic, and iron-combining properties with the corresponding properties of the same protein isolated from human and guinea pig milk.
Journal ArticleDOI
An iron-binding protein common to many external secretions
TL;DR: The iron-binding protein characteristic of milk, here called lactoferrin, has been demonstrated to occur in saliva, nasal secretions, tears, bronchial mucus, hepatic bile, pancreatic juice, seminal fluid, female cervical mucus and urines and it is suggested that it is of value in the defence of epithelial surfaces against infection.
Journal ArticleDOI
Origin of granules in polymorphonuclear leukocytes. Two types derived from opposite faces of the Golgi complex in developing granulocytes.
TL;DR: The results indicate that inversion of the azurophil/specific granule ratio occurs during the myelocyte stage and is due to: (a) reduction of azuro phil granules by multiple mitoses; (b) lack of new azuroPhil granule formation after the progranulocyte stage; and (c) continuing specific granule production.