Association of mitochondria with microtubules in cultured cells.
TLDR
By indirect immunofluorescence techniques, microtubules and mitochondria were localized in normal rat kidney cells, human WI38 fibroblasts, mouse peritoneal macrophages, and a putative smooth muscle rat cell line, in monolayer culture, suggesting that mitochondria are directly or indirectly associated with microtubule in these cells.Abstract:
By indirect immunofluorescence techniques, microtubules and mitochondria were localized in normal rat kidney cells, human WI38 fibroblasts, mouse peritoneal macrophages, and a putative smooth muscle rat cell line, in monolayer culture. The mitochondria were found to be arranged along the cytoplasmic microtubules in each cell type. Disruption of the microtubules with colcemid caused a redistribution of the mitochondria in these cells. There was no correlation between the location of the mitochondria and actin-containing filaments. This evidence suggests that mitochondria are directly or indirectly associated with microtubules in these cells.read more
Citations
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Localization of mitochondria in living cells with rhodamine 123
TL;DR: With the use of rhodamine 123, it is possible to detect alterations in mitochondrial distribution following transformation by Rous sarcoma virus and changes in the shape and organization of mitochondria induced by colchicine treatment.
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Dynamics of mitochondria in living cells: Shape changes, dislocations, fusion, and fission of mitochondria
J. Bereiter‐Hahn,M. Vöth +1 more
TL;DR: Emphasis is laid on the methods for visualizing mitochondria in cells and following their behaviour, and Fluorescence methods provide unique possibilities because of their high resolving power and because some of the mitochondria‐specific fluorochromes can be used to reveal the membrane potential.
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Mitochondrial dynamics and inheritance during cell division, development and disease.
Prashant Mishra,David C. Chan +1 more
TL;DR: Mechanisms have evolved to regulate mitochondrial segregation during cell division, oogenesis, fertilization and tissue development, as well as to ensure the integrity of these organelles and their DNA, including fusion–fission dynamics, organelle transport, mitophagy and genetic selection of functional genomes.
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Characterization of the human heart mitochondrial proteome.
Steven W. Taylor,Eoin Fahy,Bing Zhang,Gary M. Glenn,Dale E. Warnock,Sandra E. Wiley,Anne N. Murphy,Sara P. Gaucher,Roderick A. Capaldi,Bradford W. Gibson,Soumitra S. Ghosh +10 more
TL;DR: An extensive catalogue of the mitochondrial proteome using highly purified mitochondria from normal human heart tissue and subsequent detection by mass spectrometry and rigorous bioinformatic analysis yielded 615 distinct protein identifications.
Journal ArticleDOI
Microtubules and the endoplasmic reticulum are highly interdependent structures.
TL;DR: It is concluded that microtubules and the ER are highly interdependent in two ways: polymerization of individual micro Tubules and extension of individual ER tubules occur together at the level of resolution of the fluorescence microscope, and depolymerization of micro Tubule leads to a slow retraction of the ER network towards the cell center, indicating that over longer periods of time, the extended state of the entire ER network requires the microtubule system.
References
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Microtubule Assembly in the Absence of Added Nucleotides
TL;DR: Tubulin can be purified from guinea pig brain readily and in good yield by two cycles of assembly in glycerol-containing solutions, and is more stable than tubules formed in the absence of these compounds.
Journal Article
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Douglas B. Murphy,Gary G. Borisy +1 more
TL;DR: It is demonstrated that the HMW associates with microtubules as a structural component projecting from the surface of the microtubule wall by facilitating the formation of ring structures which are apparently intermediates in polymerization.
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Characterization of two putative smooth muscle cell lines from rat thoracic aorta
B W Kimes,B L Brandt +1 more
TL;DR: It is concluded that these cell lines proliferate as myoblasts and develop into cells which phenotypically resemble smooth muscle.
Journal ArticleDOI
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TL;DR: Tubulin Structure and Interaction of Tubulin with Small Molecules: Subunit Structure and Binding to Other Macromolecules.