Journal ArticleDOI
Asymbiotic seed germination and in vitro seedling development of Cyrtopodium punctatum: a propagation protocol for an endangered Florida native orchid
TLDR
This asymbiotic seed germination protocol for C. punctatum will facilitate future reintroduction projects involving this endangered species and help secure its survival in the wild.Abstract:
Cyrtopodium punctatum Lindley is an endangered epiphytic orchid restricted in the United States to southern Florida. Due to its ornamental value, the species was extensively collected from the wild during the past 100 years. Today, only a few plants remain in protected areas. As part of a conservation plan for the species, procedures for asymbiotic seed germination were developed. Five asymbiotic orchid seed germination media (PhytoTechnology Orchid Seed Sowing Medium, Knudson C, Malmgren Modified Terrestrial Orchid Medium, Vacin & Went Modified Orchid Medium, and ½-strengh Murashige & Skoog) were examined for their effectiveness in promoting seed germination and protocorm development under a 16/8 h L/D photoperiod and dark (0/24 h L/D). The influence of photoperiod on growth and development was also examined. Seeds were germinated under a 16/8 h, 12/12 h, 8/16 h L/D photoperiod, at 25 ± 3°C and allowed to develop in vitro for 10 weeks. After 10 weeks, developing seedlings were transferred to Sigma Phytatrays and returned to their assigned photoperiod treatments for continued seedling development for an additional 15 weeks. Highest germination occurred in 0/24 h L/D on PhytoTechnology Orchid Seed Sowing Medium and seedlings displayed more advanced development when cultured under 16/8 h L/D photoperiod after 15 weeks in Phytatrays. Thirty-five week old seedlings potted in coconut husk growing medium exhibited 90% survival following 5 weeks acclimatization to greenhouse conditions. This asymbiotic seed germination protocol for C. punctatum will facilitate future reintroduction projects involving this endangered species.read more
Citations
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Journal ArticleDOI
Germination and seedling establishment in orchids: a complex of requirements
TL;DR: A better understanding of germination and seedling establishment is needed for conservation of orchid populations, because the germination niches in orchid species are extremely complex and varied.
VIEWPOINT: PART OF A HIGHLIGHT ON ORCHID BIOLOGY Germination and seedling establishment in orchids: a complex of requirements
TL;DR: In this article, a mycobiont that the seedling can parasitize is considered an essential element, and a great diversity of Basidiomycota and Ascomycota have been identified for their role in orchid seed germination, with fungi identifiable as imperfect Rhizoctonia species predominating.
Journal ArticleDOI
Asymbiotic seed germination, mass propagation and seedling development of Vanda coerulea Griff ex.Lindl. (Blue Vanda): An in vitro protocol for an endangered orchid
TL;DR: A reliable protocol was developed for in vitro seed germination, protocorm multiplication and subsequent plantlet regeneration of Vanda coerulea, an endangered orchid species, and this protocol will enable mass propagation and conservation of this exquisite orchid.
Journal ArticleDOI
Mycorrhizal compatibility and symbiotic seed germination of orchids from the Coastal Range and Andes in south central Chile.
Hector Herrera,Rafael Borges da Silva Valadares,Domingo Contreras,Yoav Bashan,César Arriagada +4 more
TL;DR: In this study, isolated mycorrhizal fungi had the ability to induce seed germination at different efficiencies and with low specificity, and their widespread occurrence in some orchids suggests a putative role in plant establishment.
Journal ArticleDOI
Photoinhibition of seed germination: occurrence, ecology and phylogeny
TL;DR: Results clearly indicate that PISG is a physiological adaptation to avoid germination on the soil surface, where conditions are not suitable for seedling establishment.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Book
The Orchids: Natural History and Classification
TL;DR: A detailed examination of the structure, classification, evolution, and ecology of the Orchidaceae can be found in this paper, with 95 color photographs and 99 line drawings emphasizing orchid morphology.
Journal ArticleDOI
Some pH Changes in Nutrient Solutions
Emil F. Vacin,F. W. Went +1 more
TL;DR: An investigation indicated that amino acids are excellent buffers and a new nutrient solution is proposed, based on the results of studies on weakly buffered culture media used for asymbiotic germination of orchid seeds.
Journal ArticleDOI
Factors affecting the germination of orchid seeds
TL;DR: The nature of the factors required by germinating orchid seeds and the effects of miscellaneous factors are discussed.