Journal ArticleDOI
Automatic classification of two-dimensional gel electrophoresis pictures by heuristic clustering analysis: a step toward machine learning.
Ron D. Appel,Denis F. Hochstrasser,Christian Maurice Roch,Matthieu Funk,A F Muller,Christian Pellegrini +5 more
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TLDR
The classification method and the preliminary results obtained with liver biopsy electrophoretograms are described and heuristic clustering is also compared to other classification techniques.Abstract:
The interpretation of two-dimensional gel electrophoresis (2-DGE) profiles can be facilitated by artificial intelligence and machine learning programs. We have incorporated into our 2-DGE computer analysis system (termed MELANIE-Medical Electrophoresis Analysis Interactive Expert system) a program which automatically classifies 2-DGE patterns using heuristic clustering analysis. This program is a step toward machine learning. In this publication, we describe the classification method and the preliminary results obtained with liver biopsy electrophoretograms. Heuristic clustering is also compared to other classification techniques.read more
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Proteome and proteomics: New technologies, new concepts, and new words
TL;DR: Quantitative analysis of protein expression data obtained by high‐throughput methods has led to define the concept of “regulatory homology” and use it to begin to elucidate the basic structure of gene expression control in vivo.
Journal ArticleDOI
Two‐dimensional electrophoresis of proteins: An updated protocol and implications for a functional analysis of the genome
Joachim Klose,Ursula Kobalz +1 more
TL;DR: It is shown how effectively 2‐DE of high resolution and reproducibility can be used to study the genetic variability of proteins in an interspecific mouse backcross established by the European Backcross Collaborative Group for mapping the mouse genome.
Journal ArticleDOI
Two-dimensional gel electrophoresis in proteomics: Past, present and future.
TL;DR: A historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist to a description of the now well-known limitations of two- dimensional gels in proteomics.
Journal ArticleDOI
Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining
TL;DR: Six new crosslinking agents were synthesized and tested and diacrylylpiperazine provided increased physical strength, improved electrophoretic separation of proteins, and silver staining detection of proteins with reduced background stain.
Journal ArticleDOI
Multiple elevations of cytosolic-free Ca2+ in human neutrophils: initiation by adherence receptors of the integrin family.
TL;DR: Results suggest that following sequence of events after contact of neutrophils with surfaces: (a) cell movement and shape change lead to enhanced contact of integrins with the surface; and (b) Integrins-mediated adhesion generates multiple [Ca2+]i transients.
References
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Journal ArticleDOI
Two-dimensional gel electrophoresis of proteins.
TL;DR: The high-resolution capacity of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) makes it an excellent tool for the analysis and characterisation of complex protein mixtures and some of the biomedical applications to screening of body fluids, genetic diseases, inborn errors of metabolism, cancer and neoplastic transformation are discussed.
Book ChapterDOI
CHAPTER 2 – The QUEST System for Computer-Analyzed Two-Dimensional Electrophoresis of Proteins
Journal ArticleDOI
Design and implementation of a prototype Human Protein Index.
TL;DR: Information-handling aspects of the TYCHO I analysis system, which analyzes two-dimensional electrophoresis gels, matches the individual protein spots with those in a reference pattern, and stores various information--including spot measurements, identifications, treatment profiles, set memberships, and comments--in a computerized database are described.
Journal ArticleDOI
HERMeS: A second generation approach to the automatic analysis of two‐dimensional electrophoresis gels. Part V: Data analysis
TL;DR: It is shown that the transfer function characteristics of the input system must be carefully checked to avoid any image distortion; an easily computed criterion to obtain setting conditions giving a correct system response is proposed.
Journal ArticleDOI
An approach to completely automatic comparison of two-dimensional electrophoresis gels.
TL;DR: An attempt is being made to develop a completely automatic program for use in genetic studies, which will compare a sequence of three gels run on samples from father, mother, and child to determine whether the remaining graph differences are real or result from quantitative variation causing spots to fall below threshold.