Journal ArticleDOI
β-Cyanoethyl N,N-dialkylamino/N-morpholinomonochloro phosphoamidites, new phosphitylating agents facilitating ease of deprotection and work-up of synthesized oligonucleotides
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TLDR
In this article, β-Cyanoethyl monochlorophosphoamidites of the secondary amines N, N-dmethylamine, N,N-diisopropylamine and morpholine have been prepared which showed quantitative phosphitylation of the protected deoxynucleosides.About:
This article is published in Tetrahedron Letters.The article was published on 1983-01-01. It has received 279 citations till now. The article focuses on the topics: Morpholine & Diisopropylamine.read more
Citations
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Journal ArticleDOI
Synthesis of Oligodeoxyribonucleotides Involving a Rapid Procedure for Removal of Base-Protecting Groups by Use of the 4,4′,4″-Tris(benzoyloxy)trityl (TBTr) Group
TL;DR: In this article, a building unit for oligodeoxyribonucleotide synthesis in the phosphoramidite approach was described and the efficiency of condensation was discussed in detail.
Journal ArticleDOI
A novel phosphitylating reagent for in situ generation of deoxyribonucleoside phosphoramidites
Zhang Zhaoda,Tang Jin Yan +1 more
TL;DR: In this paper, a new reagent, 2-cyanoethoxy(N,N-diisopropylamino)3-nitro-1,2,4-triazolylphosphine (1), has been used in in in situ generation of 5′-DMT-nucleoside phosphoramidites and automated syntheses of oligonucleotides.
Journal ArticleDOI
Triplex-mediated cleavage of DNA by 1,10-phenanthroline-linked 2'-O-methyl RNA
TL;DR: The design, synthesis, and DNA cleaving activity of 1,10‐phenanthroline (OP)‐linked 2′‐O‐methyl RNA (OP‐m) is described, which found to work as preferential purine‐strand cutters for duplex DNA, they would be useful as unique tools for genome analysis.
Journal ArticleDOI
Studies in the Solid Phase Synthesis of Oligo- and Poly-Ribonucleotides
TL;DR: In this paper, solid phase synthesis of the 3′-terminal 10-mer, 19-mer and 37-mer sequences of unmodified yeast tRNAAla is described.
Journal ArticleDOI
Incorporation of 3′-C-(Hydroxymethyl)thymidine into novel oligodeoxynucleotide analogues
TL;DR: 3′-C-(Hydroxymethyl)thymidine has been incorporated into novel oligodeoxynucleotide analogues containing extended backbones using the phosphoramidite building block 4 and exhibited minor decreases in the affinity towards complementary DNA.
References
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Journal ArticleDOI
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
TL;DR: In this article, the development of a new class of nucleoside phosphites is described, which are stable to normal laboratory conditions, are activated by mild acid treatment, and are observed to react essentially quantitatively with protected nucleosides.
Journal ArticleDOI
An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotides
L.J. McBride,Marvin H. Caruthers +1 more
TL;DR: In this article, various deoxynucleoside N,N-dialkylaminomethoxyphosphines were examined for stability and reactivity in deoxyoligonucleotide synthesis.
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Hindered dialkylamino nucleoside phosphite reagents in the synthesis of two DNA 51-mers
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Letter: Phosphite coupling procedure for generating internucleotide links.
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DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in plyacrylamide-gels
TL;DR: A reliable identification of oligonucleotides on acrylamide-gels by exact chain length determination with respect to base composition and furthermore a detailed interpretation of complex reaction mixtures is presented.
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Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotides
L.J. McBride,Marvin H. Caruthers +1 more