Journal ArticleDOI
β-Cyanoethyl N,N-dialkylamino/N-morpholinomonochloro phosphoamidites, new phosphitylating agents facilitating ease of deprotection and work-up of synthesized oligonucleotides
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TLDR
In this article, β-Cyanoethyl monochlorophosphoamidites of the secondary amines N, N-dmethylamine, N,N-diisopropylamine and morpholine have been prepared which showed quantitative phosphitylation of the protected deoxynucleosides.About:
This article is published in Tetrahedron Letters.The article was published on 1983-01-01. It has received 279 citations till now. The article focuses on the topics: Morpholine & Diisopropylamine.read more
Citations
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The phosphorothioite method 2. Use of S-(t-butyl) β-cyanoethyl phosphorothioites of deoxyribonucleoside for the synthesis of oligodeoxyribonucleosides on polymer supports
TL;DR: S-(t-Butyl) β-cyanoethyl 3′-phosphorothioites of appropriately protected deoxyribonucleosides were prepared by the use of (t-butylthio) β -cyanoethoxychlorophosphine as a new phosphitylating reagent.
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Phosphorothioate: β-Fragmentierung gegen β-Silicium-Effekt†
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Preparation of rabbit antibodies to 4,4'-dimethoxytriphenylmethyl, the protective group in oligonucleotide synthesis.
Alberto Chersi,Teresa Francesca Romano,Michele Evangelista,Anna Maria Mileo,Giuliana Falasca +4 more
TL;DR: One of the two antisera appeared to recognize the dimethoxytrityl group bound to carrier molecules, and thus might find a useful application for the detection, quantitation, and control of oligonucleotides obtained by automatic synthesis.
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Synthesis of a wild-type and three mutant Cucurbita maxima trypsin inhibitor-encoding genes by a single-strand approach.
TL;DR: A single-strand approach to gene assembly, based on a modification of an in vitro complementary oligodeoxyribonucleotide template-directed ligation of the desired sequence to a linearized vector, is described.
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The preparation of a 3'-(2-cyanoethyl)phosphoramidite of 5'-o-(3-thiopropyl)methylphosphorylthymidine
TL;DR: A high-yielding three-step reaction sequence to a useful novel phosphoramidite, using 3′-O-acetylthymidine as starting material, is reported.
References
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Journal ArticleDOI
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
TL;DR: In this article, the development of a new class of nucleoside phosphites is described, which are stable to normal laboratory conditions, are activated by mild acid treatment, and are observed to react essentially quantitatively with protected nucleosides.
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An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotides
L.J. McBride,Marvin H. Caruthers +1 more
TL;DR: In this article, various deoxynucleoside N,N-dialkylaminomethoxyphosphines were examined for stability and reactivity in deoxyoligonucleotide synthesis.
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Hindered dialkylamino nucleoside phosphite reagents in the synthesis of two DNA 51-mers
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Letter: Phosphite coupling procedure for generating internucleotide links.
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DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in plyacrylamide-gels
TL;DR: A reliable identification of oligonucleotides on acrylamide-gels by exact chain length determination with respect to base composition and furthermore a detailed interpretation of complex reaction mixtures is presented.
Related Papers (5)
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotides
L.J. McBride,Marvin H. Caruthers +1 more