Journal ArticleDOI
Bile acid glucuronides, II[1]. Isolation and identification of a chenodeoxycholic acid glucuronide from human plasma in intrahepatic cholestasis.
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TLDR
From comparison of the chromatogrphic behaviour and the mass spectrum of the natural compound and the synthetic product the structure of the bile acid derivative isolated from plasma could be established as a peracetylated methylester of chenodeoxycholic acid-3-beta-D-glucuronide.Abstract:
The isolation of a glucurono-conjugate of a bile acid has been performed from human plasma in a case of chronic intrahepatic cholestasis. By means of a series of chromatographic steps, esterification with diazomethane and acetylation a mixture of methylester polyacetates of steroid glucuronides was obtained, which could be separated by thin-layer chromatography. Methyl 7alpha-acetoxy-3alpha-O-(methyl 2, 3, 4-tri-O-acetyl-beta-D-glucopyranosyluronate)5beta-cholan-24-oate, synthesized via the Koenigs-Knorr condensation reaction, was used as reference substance. From comparison of the chromatogrphic behaviour and the mass spectrum of the natural compound and the synthetic product the structure of the bile acid derivative isolated from plasma could be established as a peracetylated methylester of chenodeoxycholic acid-3-beta-D-glucuronide.read more
Citations
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Journal ArticleDOI
The hydrophobic-hydrophilic balance of bile salts. Inverse correlation between reverse-phase high performance liquid chromatographic mobilities and micellar cholesterol-solubilizing capacities.
TL;DR: The reverse-phase high performance liquid chromatographic mobilities and equilibrium cholesterol-solubilizing capacities are inverse functions of each other and correlate closely with the hydrophilicity of bile salt molecules.
Journal ArticleDOI
FXR induces the UGT2B4 enzyme in hepatocytes: a potential mechanism of negative feedback control of FXR activity.
Olivier Barbier,Inés Pineda Torra,Audrey Sirvent,Thierry Claudel,Christophe Blanquart,Daniel Duran-Sandoval,Folkert Kuipers,V. A. Kosykh,Jean-Charles Fruchart,Bart Staels +9 more
TL;DR: It is suggested that UGT2B4 gene induction by bile acids contributes to a feed-forward reduction of bile acid toxicity and a decrease of the activity of these biological FXR activators.
Journal ArticleDOI
Bile acid profiles in urine of patients with liver diseases
A. Bremmelgaard,J. Sjövall +1 more
TL;DR: Quantitative gas chromatography‐mass spectrometry was used to study the metabolic profiles of unconjugated, conjugated and sulphated bile acids in urine of patients with intermittent intrahepatic cholestasis of unknown aetiology, cirrhosis of the liver, primary biliary cirrhotic disease, viral and toxic hepatitis and extrahepasis.
Journal ArticleDOI
Peroxisome Proliferator-activated Receptor α Induces Hepatic Expression of the Human Bile Acid Glucuronidating UDP-glucuronosyltransferase 2B4 Enzyme
Olivier Barbier,Daniel Duran-Sandoval,Inés Pineda-Torra,V. A. Kosykh,Jean-Charles Fruchart,Bart Staels +5 more
TL;DR: UGT2B4 is identified as a novel target gene of the nuclear receptor peroxisome proliferator-activated receptor α (PPARα), which mediates the hypolipidemic action of fibrates and demonstrates that PPARα agonists may control the catabolism of cytotoxic bile acids.
Book ChapterDOI
Mass Spectrometry of Bile Acids
TL;DR: The continued use over many years of mass spectrometry for bile acid analysis can be attributed to its value as a method for providing definitive qualitative and quantitative information.
References
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Journal ArticleDOI
Effect of sodium taurolithocholate on bile flow and bile acid exeretion.
Norman B. Javitt,Sidney Emerman +1 more
TL;DR: Infusion of sodium taurocholenate, a nonhemolytic bile salt, caused an effect on bile flow and bile acid salt excretion qualitatively similar to Sodium taurolithocholate, and the induction of cholestasis can be attributed to the physical properties of these poorly water soluble bile salts.
Journal ArticleDOI
Steroid Metabolism in Rats Given [1‐2H2]Ethanol
TL;DR: The results indicate that deuterium is transferred from ethanol via NADH to the NADPH that is utilized in the biosynthesis of cholesterol and bile acids, and the increased production of NADH during ethanol metabolism did not induce a formation of 3β-hydroxycholanoic acids.
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