Showing papers in "Journal of Lipid Research in 1977"

TL;DR: Analysis of metabolic profiles of bile acids in urine may be a useful method in studies of the function of organs involved in bile acid metabolism.

TL;DR: Two-dimensional immunoelectrophoretic procedure was adapted and the observations of increased arginine-rich apoprotein in the B-VLDL, intermediate fraction, and HDL(c) following cholesterol feeding remained valid, however, precise quantitation awaits refinements in lipoprotein isolation techniques.

TL;DR: It is concluded that Lp(a) is present in all individuals with apolipoprotein B and that apoliprotein B appears necessary for the plasma transport of the Lp (a) lipoprotein.

TL;DR: The results suggest that many individuals have "remnant" very low density lipoproteins in their plasma, however, the beta-migrating " Remnant" that accumulated in large amounts in individuals with primary dysbetalipoproteinemia contains much more arginine-rich protein and this protein is structurally abnormal.

TL;DR: Analysis of cholesteryl ester specific activity-time curves for the very low density lipoproteins subfraction from hypercholesterolemic plasma showed that nearly all esterified cholesterol in large very lowdensity lipoproteinins and approximately 30% of esterify cholesterol in small very lowensity lipopoteins was derived from chylomicron degradation.

TL;DR: Glycine and taurine conjugates of 5beta-cholanic acids have been synthesized using improved procedures based on the peptide coupling reagent, N-ethoxycarbonyl-2-eth oxygen-1,2-dihydroquinoline in yields higher than 90%.

TL;DR: Observations indicate that nascent chylomicrons accumulate within Golgi vesicles as a pre-requisite to secretion and that secretion occurs by exocytosis resulting in the release of nascent chlyomicrons from secretory vesicle-membrane fusion.

TL;DR: A quantitative high performance liquid chromatography method for the analysis of neutral glycosylceramides as their perbenzoyl derivatives has been devised and has been applied to the quantitative analysis of erythrocyte and plasma glycolipids.

TL;DR: The properties of the high density lipoproteins produced in this system are remarkably similar to those found in humans with genetically determined deficiency of lecithin-cholesterol acyltransferase.

TL;DR: The data support the concept that during normal development a "bed" of preadipocytes is synthesized during the suckling period and suggest a regulatory role for lipoprotein lipase in the control of "lipid-filling" during early postnatal development.

TL;DR: The increase in LDL molecular weight was associated with a large increase in cholesteryl ester content and concomitant smaller increases in protein, phospholipid, and free cholesterol as molecular weight increased, and these components appeared to be added to the LDL particles together as discrete increments of fixed composition.

TL;DR: There was no loss of radioactivity from the fixed cells with hydrogen peroxide treatment, and in the smaller-sized cell ranges there was a significant reduction in apparent adipocyte number of rat, pig, and beef adipose tissue with 8 M urea treatment.

TL;DR: Diphenyl carbazide containing diphenylcarbazone is used as the color developing reagent instead of diethyldithiocarbamate which was employed in the previous method.

TL;DR: Analytical isoelectric focusing (IEF) has been applied to the study of the apolipoprotein components of rat serum high density and very low density lipoproteins and tends to rule out carbamylation or incomplete unfolding of the proteins in the presence of urea as the causes of the observed heterogeneity.

TL;DR: Findings imply that, at most, only a small fraction of bound HDL binds to the high-affinity LDL receptor and/or that HDL binding there is internalized very slowly, suggesting that peripheral tissues may contribute to HDL catabolism.

TL;DR: The intestinal perfusion method appears to provide certain advantages over previous techniques for estimating total cholesterol entering the upper intestine, including that derived from both endogenous and exogenous sources.

TL;DR: The results indicate that dietary modification of the microsomal fatty acid composition is associated with alterations in the activity of ACAT, an enzyme that is tightly bound to theMicrosomes, which may be partly responsible for the differences in cholesteryl ester contents of Ehrlich cells grown in mice fed the coconut and sunflower oil diets.

TL;DR: Similarities between triolein and cholesteryl oleate hydrolysis existed with respect to pH optimum and the effect of cholesterol feeding on activity, suggesting that a single enzyme may hydrolyze both lipids.

TL;DR: Comparisons between normal RBC and vitamin E-deficient RBC suggest that vitamin E has little effect on the inward diffusion of extra-cellular H(2)O(2).

TL;DR: A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceridelipase (H-TGL) and lipoprotein lipase (LPL); recoveries of 74 and 97% of these enzyme activities, respectively.

TL;DR: An improved method for the partial chemical synthesis of unsaturated and radioactively labeled phosphatidylcholines is described and sn-glycero-3-phosphorylcholine is reacted with twice the theoretical amount of fatty acid imidazolide and sodium methylsulfinylmethide in dimethylsulfoxide for several minutes at 17 degrees C.

TL;DR: The effects of aqueous systems suggest the possibility of cholesterol precipitation from dietary fat when it becomes mixed with water in the diet or stomach, or with the micellar phase in the intestine.

TL;DR: The method proved successful in the separation of the serum lipoproteins of the few patients with dyslipoproteinemia (abetalipoproteinemi and familial hypercholesterolemia) who were examined and indicates that the single-spin method could be useful in clinical studies as a complement to other established techniques.

TL;DR: The effects of ethynylestradiol on endogenous bile acids, their capacity to conjugate and excrete intravenously infused cholic acid, the concentrations of biliary cholesterol and lecithin, and the individual molecular species of phosphatidylcholine have been determined in male and female Sprague-Dawley rats.

TL;DR: Lipoprotein lipase mediates the removal of plasma triglycerides in extrahepatic tissues and its activity in adipose tissue and heart has been extensively studied but that in skeletal muscles has not.

TL;DR: Sterol metabolism studies using a combination of isotopic and chromatographic procedures were carried out in rats fed either a low-cholesterol stock diet or a stock diet containing 0.1% cholesterol, showing a decrease in acidic steroid synthesis, cholesterol turnover, and cholesterol balance, compared to controls.

TL;DR: Evidence is presented suggesting that a single enzyme is responsible for both catalytic activities of lysophospholipase and transacylase, and this enzyme was capable of synthesizing disaturated phosphatidylcholine, an important component of the pulmonary surfactant.

TL;DR: It is concluded that lysobisphosphatidic acid was enriched in the lysosomes of the BHK cells and that the lYSosomes also contained variable amounts of neutral lipids in the form of intralysosomal droplets.

TL;DR: Adipose tissue, from two depots in pigs of three breeding groups with different propensities to fatten, was characterized in terms of weight of the adipose tissue organ, adipose cell number, and mean cell volume as determined by electronic counting of adipose cells fixed with osmium tetroxide.