Journal ArticleDOI
Biosensing and bioremediation of Cr(VI) by cell free extract of Enterobacter aerogenes T2.
Jigisha Panda,Priyabrata Sarkar +1 more
TLDR
Calcium alginate beads, modified with CFE of E. aerogenes, could be used in bioremediation of Cr(VI) since it could work in real conditions with extraordinarily high capacity and could be a viable alternative to costly measurement procedures.Abstract:
Hexavalent chromium or Cr(VI) enters the environment through several anthropogenic activities and it is highly toxic and carcinogenic. Hence it is required to be detected and remediated from the environment. In this study, low-cost and environment-friendly methods of biosensing and bioremediation of Cr(VI) have been proposed. Crude cell free extract (CFE) of previously isolated Enterobacter aerogenes T2 (GU265554; NII 1111) was prepared and exploited to develop a stable biosensor for direct estimation of Cr(VI) in waste water, by using three electrodes via cyclic voltammetry. For bioremediation studies, a homogeneous solution of commercially available sodium alginate and CFE was added dropwise in a continuously stirred calcium chloride solution. Biologically modified calcium alginate beads were produced and these were further utilized for bioremediation studies. The proposed sensor showed linear response in the range of 10-40 μg L(-1) Cr(VI) and the limit of detection was found to be 6.6 μg L(-1) Cr(VI). No interference was observed in presence of metal ions, e.g., lead, cadmium, arsenic, tin etc., except for insignificant interference with molybdenum and manganese. In bioremediation studies, modified calcium alginate beads showed encouraging removal rate 900 mg Cr(VI)/m(3) water per day with a removal efficiency of 90%, much above than reported in literature. The proposed sensing system could be a viable alternative to costly measurement procedures. Calcium alginate beads, modified with CFE of E. aerogenes, could be used in bioremediation of Cr(VI) since it could work in real conditions with extraordinarily high capacity.read more
Citations
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Journal ArticleDOI
Three-Stage Single-Chambered Microbial Fuel Cell Biosensor Inoculated with Exiguobacterium aestuarii YC211 for Continuous Chromium (VI) Measurement.
TL;DR: A three-stage single-chambered microbial fuel cell (SCMFC) biosensor inoculated with Exiguobacterium aestuarii YC211 was developed for in situ, real-time, and continuous Cr(VI) measurement of tannery wastewater and showed potential as an early warning device with wide dynamic range.
Allium sativum aqueous extract prevents potassium dichromateinduced nephrotoxicity and lipid oxidation in rats
Sergio L Becerra-Torres,Cesar Soria-Fregozo,Fernando Jaramillo-Juárez,José L Moreno-Hernández-Duque +3 more
TL;DR: In this paper, the authors study the proteccion potencial de Allium sativum against nefrotoxicidad and oxidacion lipidica inducible by K2Cr2O7 in ratas.
Journal ArticleDOI
Highly Sensitive Luminescent Bioassay Using Recombinant Escherichia coli Biosensor for Rapid Detection of Low Cr(VI) Concentration in Environmental Water.
TL;DR: In this paper, the authors constructed a recombinant Escherichia coli strain with different promoters inserted between the chromate-sensing regulator chrB and the reporter gene luxAB to sense low hexavalent chromium (Cr(VI)) concentrations in various water bodies.
Journal ArticleDOI
Uranium Removal from Wastewater Using Immobilized Multiple Heavy-Metal and Antibiotic Resistance E. coli Isolated from Aborshid Egypt
TL;DR: In this paper, 6 bacterial isolate, isolated Uranium Ore samples from Aborshid Egypt, were characterized for their response to 15 antibiotics and 10 heavy metals, including Pb, Ni, Cu and Zn.
Allium sativum aqueous extract prevents potassium dichromate- induced nephrotoxicity and lipid oxidation in rats (El extracto acuoso de Allium sativum previene la nefrotoxicidad y la oxidación lipídica inducida por dicromato de potasio en ratas)
Sergio L Becerra-Torres,Cesar Soria-Fregozo,Fernando Jaramillo-Juárez,José L Moreno-Hernández-Duque +3 more
TL;DR: The K2Cr2O7 caused significant renal dysfunction, but Allium sativum extract prevented this condition by improving all measured biomarkers, and the supply of an aqueous extract prevented the disorders caused by this metal.
References
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Journal ArticleDOI
Chromate Reduction by Chromium-Resistant Bacteria Isolated from Soils Contaminated with Dichromate
Flávio Anastácio de Oliveira Camargo,Fatima Menezes Bento,Benedict C. Okeke,William T. Frankenberger +3 more
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Bioremediation of chromium by novel strains Enterobacter aerogenes T2 and Acinetobacter sp. PD 12 S2
Jigisha Panda,Priyabrata Sarkar +1 more