Journal ArticleDOI
Cadmium effect on oxidative metabolism of pea (Pisum sativum L.) roots. Imaging of reactive oxygen species and nitric oxide accumulation in vivo
María Rodríguez-Serrano,María C. Romero-Puertas,Ana Zabalza,Francisco J. Corpas,Manuel Gómez,Luis A. del Río,Luisa M. Sandalio +6 more
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TLDR
Analysis of reactive oxygen species (ROS) and nitric oxide (NO) levels by fluorescence and confocal laser microscopy showed an over-accumulation of O2*- and H2O2, and a reduction in the NO content in lateral and principal roots, which could regulate the cellular response to cope with damages imposed by cadmium.Abstract:
Growth of pea (Pisum sativum L.) plants with 50 microM CdCl2 for 15 d produced a reduction in the number and length of lateral roots, and changes in structure of the principal roots affecting the xylem vessels. Cadmium induced a reduction in glutathione (GSH) and ascorbate (ASC) contents, and catalase (CAT), GSH reductase (GR) and guaiacol peroxidase (GPX) activities. CuZn-superoxide dismutase (SOD) activity was also diminished by the Cd treatment, although Mn-SOD was slightly increased. CAT and CuZn-SOD were down-regulated at transcriptional level, while Mn-SOD, Fe-SOD and GR were up-regulated. Analysis of reactive oxygen species (ROS) and nitric oxide (NO) levels by fluorescence and confocal laser microscopy (CLM) showed an over-accumulation of O2*- and H2O2, and a reduction in the NO content in lateral and principal roots. ROS overproduction was dependent on changes in intracellular Ca+2 content, and peroxidases and NADPH oxidases were involved. Cadmium also produced an increase in salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) contents. The rise of ET and ROS, and the NO decrease are in accordance with senescence processes induced by Cd, and the increase of JA and SA could regulate the cellular response to cope with damages imposed by cadmium.read more
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Heavy metals in soils : trace metals and metalloids in soils and their bioavailability
TL;DR: In this article, the authors defined the sources of heavy metals and metalloids in Soils and derived methods for the determination of Heavy Metals and Metalloids in soil.
Journal ArticleDOI
The relationship between metal toxicity and cellular redox imbalance
TL;DR: Analysis of plants expressing targeted modifications of components of the antioxidant system and comparison of closely related plant species with different degrees of toxic metal sensitivity have established a link between the degree of plant tolerance to metals and the level of antioxidants.
Journal ArticleDOI
Unravelling cadmium toxicity and tolerance in plants: Insight into regulatory mechanisms
Susana M. Gallego,Susana M. Gallego,Liliana B. Pena,Liliana B. Pena,Roberto A. Barcia,Claudia E. Azpilicueta,María Florencia Iannone,María Florencia Iannone,Eliana Paola Rosales,Eliana Paola Rosales,Myriam Sara Zawoznik,María Daniela Groppa,María Daniela Groppa,Maria Patricia Benavides,Maria Patricia Benavides +14 more
TL;DR: This article reviews current knowledge of uptake, transport and accumulation of Cd in plants and gives an overview of C d-detoxification mechanisms, Cd-induced oxidative damage and antioxidant defenses in plants, and presents a picture of the role of reactive oxygen and nitrogen species in Cd toxicity.
Journal ArticleDOI
Molecular Mechanism of Heavy Metal Toxicity and Tolerance in Plants: Central Role of Glutathione in Detoxification of Reactive Oxygen Species and Methylglyoxal and in Heavy Metal Chelation
Mohammad Anwar Hossain,Mohammad Anwar Hossain,Pukclai Piyatida,Jaime A. Teixeira da Silva,Masayuki Fujita +4 more
TL;DR: The aim of this review is to integrate a recent understanding of physiological and biochemical mechanisms of HM-induced plant stress response and tolerance based on the findings of current plant molecular biology research.
Journal ArticleDOI
Cellular Response of Pea Plants to Cadmium Toxicity: Cross Talk between Reactive Oxygen Species, Nitric Oxide, and Calcium
María Rodríguez-Serrano,María C. Romero-Puertas,Diana M. Pazmiño,Pilar S. Testillano,María Carmen Risueño,Luis A. del Río,Luisa M. Sandalio +6 more
TL;DR: A model is proposed for the cellular response to long-term Cd exposure consisting of cross talk between Ca, ROS, and NO.
References
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