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Journal ArticleDOI

Carbohydrate Composition and Acid Invertase Activity in Rice Leaves Infected with Pyricularia oryzae

Byung Kook Hwang, +2 more
- 01 Feb 1989 - 
- Vol. 125, Iss: 2, pp 124-132
TLDR
The presence of arabitol only in blast-infected rice leaves suggests that arab Capitol synthesis may be induced in infected leaf tissue as a result of the rice-blast fungus interaction, but may not be within blast fungus itself.
Abstract
Ethanol-soluble carbohydrates in healthy and blast-infected leaves and also cultures of Pyricularia oryzae were analyzed using gas chromatography Arabitol, mannitol, and trehalose occurred in infected leaf tissue, but not in healthy controls Cultures of P oryzae contained mannitol and trehalose, but not arabitol and sucrose The presence of arabitol only in blast-infected rice leaves suggests that arabitol synthesis may be induced in infected leaf tissue as a result of the rice-blast fungus interaction, but may not be within blast fungus itself The amounts of glucose, fructose, and sucrose in infected leaves were slightly increased until 5 days, and greatly enhanced at 7 days after inoculation, There were no differences in amounts of these sugars between the cultivars Nakdong and Dobong At 7 days after inoculation, increases in amounts of arabitol, mannitol, and trehalose were pronounced in the susceptible cultivar Nakdong than in the moderately susceptible cultivar Dobong The increased amounts of glucose and fructose in infected plants of the two cultivars were closely correlated with the presence of a very active invertase Zusammenfassung Athanollosliche Kohlenhydrate in gesunden und mit Reisbraune infizierten Blattern und auch in Pyricularia oryzae-Kulturen wurden gaschromatographisch analysiert Arabitol, Mannitol und Trehalose waren im infizierten Blattgewebe vorhanden, aber nicht im gesunden Cewebe P oryzae-Kulturen enthielten Mannitol und Trehalose, aber kein Arabitol und Saccharose Das Vorhandensein von Arabitol nur in durch Reisbraune infizierten Blattern ist ein Hinweis dafur, das die Arabitolsynthese im infizierten Blattgewebe durch die Wechselwirkung zwischen Reis und P oryzae induziert sein kann, aber nicht innerhalb von P oryzae selbst Die Gehalte der Glukose, Fruktose und Saccharose in den infizierten Blattern waren bis funf Tage nach der Infektion etwas, aber am siebten Tage stark erhoht Die Niveaus dieses Zuckers waren zwischen den Sorten Nakdong und Dobong nicht unterschiedlich Am siebten Tage nach der Infektion waren die Zunahme von Arabitol, Mannitol und Trehalose in der anfalligen Sorte Nakdong mehr ausgepragt als in der mittelmasig anfalligen Sorte Dobong Die zunehmenden Gehalte von Glukose und Fruktose in den infizierten Pflanzen der zwei Sorten korrelierten stark mit den Invertaseaktivitatniveaus

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Citations
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Journal ArticleDOI

Independent signaling pathways regulate cellular turgor during hyperosmotic stress and appressorium-mediated plant infection by Magnaporthe grisea.

TL;DR: This work has shown that independent signal transduction pathways regulate cellular turgor during hyperosmotic stress and appressorium-mediated plant infection in Magnaporthe grisea, and that Δosm1 mutants showed a dramatically reduced ability to accumulate arabitol in the mycelium.
Journal ArticleDOI

Having a blast: exploring the pathogenicity of Magnaporthe grisea

TL;DR: Through a combination of cytology and molecular-genetic analysis, a picture is gradually emerging of the many interlinked processes that are required for successful infection of the plant.
Journal ArticleDOI

Accumulation of β-Fructosidase in the Cell Walls of Tomato Roots following Infection by a Fungal Wilt Pathogen

TL;DR: This data suggests that induction of beta-fructosidase upon fungal infection is part of the plant's defense response and the possible physiological role(s) of this enzyme in infected tomato plants is discussed in relation to the high demand in energy and carbon sources during pathogenesis.
Journal ArticleDOI

Carbohydrate composition of apoplastic fluids isolated from tomato leaves inoculated with virulent or avirulent races of Cladosporium fulvum (syn. Fulvia fulva)

TL;DR: Results suggest that, upon colonization of the intercellular spaces by virulent races ofC.
References
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Journal ArticleDOI

A photometric adaptation of the somogyi method for the determination of glucose

TL;DR: In this paper, the reliability of the various Somogyi-Shaffer-Hartmann (SHH) copper reagents for glucose determination in biological material has been established, which can be accomplished by omission of the iodide and iodate in their preparation, since these interfere with the molybdate color reagents.
Journal ArticleDOI

A colorimetric method for the determination of sugars.

TL;DR: Preliminary experiments showed that the anthrone and the α-naphthol sulphonate reagents give good results with pure sugar solutions, but the presence of only traces of solvents such as butanol, phenol and propionic acid used in the chromatographic separation of the sugars rendered them useless.
Journal ArticleDOI

The activity of invertase and its relevance to the accumulation of storage polysaccharides in leaves infected by biotrophic fungi

TL;DR: It is concluded that sucrose from the host is first hydrolysed and then absorbed by the parasite, and invertase also plays a key role in the provision of substrate for the typical accumulation of starch around pustules of biotrophic fungi on host species which store this polysaccharide.
Journal ArticleDOI

Uptake of sugars by epichloe typhina (pers. ex fr.) tul. in culture and from its host, agrostis stolonifera l.

TL;DR: Epichloe typhina forms an intercellular systemic mycelium within its host, Agrostis stolonifera, and a stroma (upon which conidia and perithecia are produced) at the junction of the lamina and sheath of the flag leaf where association between the stroma and vascular tissue of the host is close.
Journal ArticleDOI

Carbohydrate metabolism in the fungus dendryphiella salina

TL;DR: In this paper, Dendryphiella salina was grown on non-saline media, and the only polyols present in the mycelium were mannitol and arabitol.
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