Journal ArticleDOI
Catalytic Activity in Organic Solvents and Stability of Immobilized Enzymes Depend on the Pore Size and Surface Characteristics of Mesoporous Silica
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TLDR
In this paper, an enzyme, horseradish peroxidase (HRP), was adsorbed in the manner of the single immersion method on the silica mesoporous materials FSM-16, MCM-41, and SBA-15 with various pore diameters from 27 to 92 A, and their enzymatic activities in an organic solvent and thermal stabilities were studied.Abstract:
An enzyme, horseradish peroxidase (HRP), was adsorbed in the manner of the single immersion method on the silica mesoporous materials FSM-16, MCM-41, and SBA-15 with various pore diameters from 27 to 92 A, and their enzymatic activities in an organic solvent and the thermal stabilities were studied. FSM-16 and MCM-41 showed a larger amount of adsorption of HRP than SBA-15 or silica gel when the pore sizes were larger than the 50 A. The increased enzyme adsorption capacity may be due to the surface characteristics of FSM-16 and MCM-41, which would be consistent with the observed larger adsorption capacity of cationic pigment compared with anionic pigment for these materials. The immobilized HRP on FSM-16 and MCM-41 with pore diameter 50 A showed the highest enzymatic activity in an organic toluene and thermal stability in aqueous solution at the temperature of 70 °C. The immobilized enzymes on the other mesoporous materials including large or small pore sized FSM-16 showed lower enzymatic activity in an or...read more
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Ordered Mesoporous Materials for Bioadsorption and Biocatalysis
TL;DR: A growing field of amino acids, vitamins, enzymes, and whole cells adsorbed (immobilized) onto ordered mesoporous silica and carbon molecular sieves has attracted considerable attention over the past few years as discussed by the authors.
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Industrial use of immobilized enzymes
TL;DR: The development and attributes of several established and emerging industrial applications for immobilized enzymes, including high-fructose corn syrup production, pectin hydrolysis, debittering of fruit juices, interesterification of food fats and oils, biodiesel production, and carbon dioxide capture are reviewed herein, highlighting factors that define the advantages of enzyme immobilization.
Journal ArticleDOI
Periodic Mesoporous Silica-Based Organic−Inorganic Nanocomposite Materials
Abdelhamid Sayari,Safia Hamoudi +1 more
TL;DR: The area of periodic mesoporous materials prepared by cooperative assembly in the presence of amphiphile molecules underwent dramatic growth as discussed by the authors, and many types may be regrouped in the future.
Journal ArticleDOI
Nanostructures for enzyme stabilization
TL;DR: Recently reported approaches to improve the enzyme stability in various nanostructures such as nanoparticles, nanofibers, mesoporous materials, and single enzyme nanoparticles (SENs) are reviewed.
Journal ArticleDOI
Challenges and breakthroughs in recent research on self-assembly
Katsuhiko Ariga,Jonathan P. Hill,Michael V. Lee,Ajayan Vinu,Richard Charvet,Somobrata Acharya +5 more
TL;DR: In this paper, the salient aspects of self-assembly through the introduction of the recent challenges and breakthroughs in three categories: (i) types of selfassembly in bulk media; (ii) type of components for self-assembling in bulk medium; and (iii) selfassembly at interfaces.
References
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Journal ArticleDOI
Immobilized Enzymes and Cells as Practical Catalysts
TL;DR: Applications of immobilized enzymes and cells in the chemical, pharmaceutical, and food industries, in clinical and chemical analyses, and in medicine, as well as probable future trends in enzyme technology are discussed.
Journal ArticleDOI
Syntheses of Highly Ordered Mesoporous Materials, FSM-16, Derived from Kanemite
TL;DR: In this paper, the formation process of mesoporous materials from kanemite was divided into two elemental processes: i) exchange of Na+ in the interlayer of kanemites for alkyltrimethylammonium cations and ii) condensation of silicates and formation of a three dimensional silicate framework.
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Synthesis and comparative reactivity and electronic structural features of [MFe3S4]z+ cubane-type clusters (M = iron, cobalt, nickel)
TL;DR: In this article, the heterometal cubane type clusters [CoFe 3 S 45 (Smes) 4 ] 2 and [NiFeS 4 (PPh 3 )(Smes' 3 ] 2] have been prepared in good yield as Et 4 N + salts by reductive rearrangement reactions of the linear cluster with Co(I) and Ni(0) reactants, respectively.
Journal ArticleDOI
Synthesis of MCM-41 with Different Pore Diameters without Addition of Auxiliary Organics
TL;DR: In this paper, the authors suggest that the mechanism of swelling is related with the replacement of some CTMA+ species by others cations in the interface formed between the liquid crystal and the silica surfaces.
Journal ArticleDOI
Structural interactions between horseradish peroxidase C and the substrate benzhydroxamic acid determined by X-ray crystallography.
Anette Henriksen,David J. Schuller,K. Meno,Karen G. Welinder,and Andrew T. Smith,Michael Gajhede +5 more
TL;DR: The ability of BHA to bind to peroxidases, which lack a peripheral hydrophobic pocket, suggests that BHA is a general counterpart for the conserved hydrogen bond donors and acceptors of the distal catalytic site.