Cell cycle-dependent duplication and bidirectional migration of SeqA-associated DNA-protein complexes in E. coli.
TLDR
Using immunofluorescence microscopy, it is found that SeqA protein, a regulator of replication initiation, is localized as discrete fluorescent foci in E. coli wild-type cells and MukB protein is required for correct localization of SequA complexes by an unknown mechanism.About:
This article is published in Molecular Cell.The article was published on 1998-02-01 and is currently open access. It has received 194 citations till now. The article focuses on the topics: SeqA protein domain & FtsZ.read more
Citations
More filters
Journal ArticleDOI
Localization of bacterial DNA polymerase: evidence for a factory model of replication.
TL;DR: This work visualized DNA polymerase of the bacterium Bacillus subtilis in living cells by the creation of a fusion protein containing the catalytic subunit (PolC) and green fluorescent protein (GFP).
Journal ArticleDOI
Themes and variations in prokaryotic cell division
TL;DR: Although some cell division determinants such as FTSZ are present in a broad spectrum of prokaryotic species, the lack of FtsZ in some species and different profiles of cell division proteins in different families suggests that there are diverse mechanisms for regulating cell division.
Journal ArticleDOI
Regulating DNA Replication in Bacteria
TL;DR: The principle of replication-coupled negative regulation of DnaA found in E. coli is conserved in eukaryotes as well as in bacteria, where regulations by oriC-binding proteins and dnaA gene expression are also conserved.
Journal ArticleDOI
FtsZ ring clusters in min and partition mutants: role of both the Min system and the nucleoid in regulating FtsZ ring localization.
Xuan Chuan Yu,William Margolin +1 more
TL;DR: A model is proposed in which both the inhibitory effect of the nucleoid and the regulation by MinCDE ensure that cells divide precisely at the midpoint, suggesting that all positions along the cell length are competent for FtsZ ring assembly.
Journal ArticleDOI
Identification and characterization of a negative regulator of FtsZ ring formation in Bacillus subtilis.
TL;DR: It is proposed that EzrA interacts either with FtsZ or with one of its binding partners to promote depolymerization and is associated with the cell membrane and also colocalized with FTSZ to the nascent septal site.
References
More filters
Journal ArticleDOI
The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
TL;DR: The strategy was to measure the sizes of partial restriction enzyme digests by hybridization with a vector probe in a manner analogous to nucleotide sequencing, and found that this strategy is applicable to analyses of the genomes of other organisms.
Book ChapterDOI
Immunofluorescence Methods for Yeast
TL;DR: This chapter provides protocols for the application of immunofluorescence procedures to yeast and notes especially the great labor involved in visualizing the structure of whole cells by serial-section methods.
Journal ArticleDOI
SeqA: A negative modulator of replication initiation in E. coli
TL;DR: The identification of a gene required for sequestration is reported and it is demonstrated that this gene, seqA, also serves as a negative modulator of the primary initiation process, suggesting that SeqA might be a cooperativity factor, acting to make the replication initiation process dependent upon cooperative interactions among components.
Journal ArticleDOI
Telomere-led premeiotic chromosome movement in fission yeast.
Yuji Chikashige,Da-Qiao Ding,Hironori Funabiki,Tokuko Haraguchi,Shinro Mashiko,Mitsuhiro Yanagida,Yasushi Hiraoka +6 more
TL;DR: The movement of chromosomes that precedes meiosis was observed in living cells of fission yeast by fluorescence microscopy and the clustering of telomeres is reminiscent of the bouquet structure of meiotic-prophase chromosomes observed in higher eukaryotes, which suggests that telomere perform specific functions required for premeiotic chromosomal events generally in eucaryotes.
Journal ArticleDOI
E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork.
Joseph Campbell,Nancy Kleckner +1 more
TL;DR: Evidence is presented that oriC is a single function unit that is specifically sequestered from dam methyltransferase for a significant period of time and then released, and that the dnaA promoter region is subject to sequestration analogous to that observed at oriC and thus that hemimethylation-dependent sequestration is a general phenomenon.