Patent
Cell patterning technique
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TLDR
In this article, a mask is positioned adjacent to a surface to cover some portions of the surface while allowing other portions to remain uncovered, and then cells are applied to the uncovered portions and the mask removed.Abstract:
The present invention provides a masking system for selectively applying cells to predetermined regions of a surface. A mask is positioned adjacent to a surface to cover some portions of the surface while allowing other portions of the surface to remain uncovered. Cells then are applied to uncovered portions of the surface and the mask removed. Alternatively, a cell-adhesion promoter is applied to uncovered portions of the surface, and then cells are applied to the surface before or after removal of the mask from the surface. The masking system can be pre-coated, at least on those surfaces which will come into contact with cells, with a cell-adhesion inhibitor to resist absorption of cells and thereby avoid cell damage when the mask is removed (if cells are deposited prior to removal of the mask). A polymeric elastomeric mask that comes into cohesive-conformal contact with a surface to be patterned can be used.read more
Citations
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References
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Journal ArticleDOI
Geometric control of cell life and death.
TL;DR: Human and bovine capillary endothelial cells were switched from growth to apoptosis by using micropatterned substrates that contained extracellular matrix-coated adhesive islands of decreasing size to progressively restrict cell extension.
Patent
Microfabricated elastomeric valve and pump systems
TL;DR: In this article, a method of fabricating an elastomeric structure, comprising of forming a first layer on top of a first micromachined mold, the second layer having a second raised protrusion which formed a first recess extending along a bottom surface of the first layer, was proposed.
Patent
Multi-array, multi-specific electrochemiluminescence testing
Jacob N. Wohlstadter,James L. Wilbur,George Sigal,Mark Martin,Liang-Hong Guo,Alan B. Fischer,Jon Leland +6 more
TL;DR: In this article, a patterned multi-array, multi-specific surfaces for use in diagnostics are presented for detecting or measuring an analyte of interest using electrochemiluminescence methods.
Patent
Formation of microstamped patterns on surfaces and derivative articles
Amit Kumar,George M. Whitesides +1 more
TL;DR: In this article, a method of patterning a material surface is provided in which an elastomeric stamp having a stamping surface is coated with a self-assembled monolayer forming species having a functional group selected to bind to a particular material.
Journal ArticleDOI
Using microcontact printing to pattern the attachment of mammalian cells to self-assembled monolayers of alkanethiolates on transparent films of gold and silver
TL;DR: A convenient methodology for patterning substrates for cell culture that allows the positions and dimensions of attached cells to be controlled and an experimentally simple, nonphotolithographic process to pattern the formation of SAMs at the micrometer scale is described.