Journal ArticleDOI
Cell-surface display of E7 antigen from human papillomavirus type-16 in Lactococcus lactis and in Lactobacillus plantarum using a new cell-wall anchor from lactobacilli.
Naima G. Cortes-Perez,Vasco Azevedo,Juan Manuel Alcocer-González,Cristina Rodríguez-Padilla,Reyes Tamez-Guerra,Gérard Corthier,Alexandra Gruss,Philippe Langella,Luis G. Bermúdez-Humarán +8 more
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TLDR
The human papillomavirus type-16 E7 protein is considered a major viral oncoprotein involved in cervical cancer and a potential candidate for the development of a vaccine against this neoplasia and two lactic acid bacteria were engineered to deliver an E7 mutant protein, which could fit better to therapeutic use in humans than the native form of E7.Abstract:
The human papillomavirus type-16 (HPV-16) E7 protein is considered a major viral oncoprotein involved in cervical cancer (CxCa) and a potential candidate for the development of a vaccine against this neoplasia. Here, two lactic acid bacteria (the model one Lactococcus lactis and a probiotic one Lactobacillus plantarum) were engineered to deliver an E7 mutant protein (E7mm), which has a reduced transforming activity and consequently, could fit better to therapeutic use in humans than the native form of E7. An efficient cell-surface display of E7mm was obtained in L. lactis using an expression cassette encoding a precursor composed of (i) the signal peptide and the first 15 amino acids of the mature part of the lactococcal Usp45 protein; (ii) E7mm and (iii) the cell-wall anchor of the Streptococcus pyogenes M6 protein (CWA(M6)). This hybrid precursor was produced but not cell-wall anchored in Lb. plantarum. We thus replaced CWA(M6) by the cell-wall anchor of a Lb. plantarum protein which allows an efficient cell-wall anchoring of E7mm in this bacterium. The E7mm production and cell-surface display in both L. lactis and a probiotic bacterium, Lb. plantarum, represent one more step towards the development of a safe and effective treatment against CxCa.read more
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Therapeutic human papillomavirus vaccines: current clinical trials and future directions
TL;DR: HPV therapeutic vaccines may provide a potentially promising approach for the control of lethal HPV-associated malignancies, with emphasis on current progress of HPV vaccine clinical trials.
Journal ArticleDOI
Perspectives for therapeutic HPV vaccine development
TL;DR: Great progress has been made to develop and improve novel therapeutic HPV vaccines to treat existing HPV infections and diseases; however, there is still much work to be done.
Journal ArticleDOI
Intragastric administration of a superoxide dismutase-producing recombinant Lactobacillus casei BL23 strain attenuates DSS colitis in mice.
Laurie Watterlot,Tatiana Rochat,Harry Sokol,Claire Cherbuy,Ismael Bouloufa,François Lefèvre,Jean-Jacques Gratadoux,Edith Honvo-Hueto,Stefan Chilmonczyk,Sébastien Blugeon,Gérard Corthier,Philippe Langella,Luis G. Bermúdez-Humarán +12 more
TL;DR: Results suggest that Lb.
Journal ArticleDOI
Immunotherapy for cervical cancer: Research status and clinical potential.
Jun Han Su,Anjui Wu,Elizabeth Scotney,Barbara Ma,Archana Monie,Chien Fu Hung,Chien Fu Hung,Tzyy Choou Wu,Tzyy Choou Wu +8 more
TL;DR: The HPV-encoded early proteins, the E6 and E7 oncoproteins, form ideal targets for therapeutic HPV vaccines, since they are consistently expressed in HPV-associated cervical cancer and its precursor lesions and thus play crucial roles in the generation and maintenance of HPV- associated disease.
Journal ArticleDOI
Perspectives for Preventive and Therapeutic HPV Vaccines
TL;DR: Current preventive and therapeutic HPV vaccines and their future directions are discussed, which aim to generate a cell-mediated immune response to infected cells.
References
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Journal ArticleDOI
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