Journal ArticleDOI
Characterisation of methacrylate monoliths for bacteriophage purification.
TLDR
Binding of three different bacteriophages (phages), namely T7, lambda and M13 on methacrylate monoliths was investigated and under optimal conditions, recovery for all three phages approached 100%.About:
This article is published in Journal of Chromatography A.The article was published on 2011-04-29. It has received 49 citations till now. The article focuses on the topics: Bacteriophage & T7 phage.read more
Citations
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Journal ArticleDOI
Considerations for the Use of Phage Therapy in Clinical Practice
Gina A. Suh,Thomas P. Lodise,Pranita D. Tamma,Jane M. Knisely,J. Alexander,Saima Aslam,Karen D. Barton,Erica Bizzell,Katherine M. C. Totten,Joseph L. Campbell,Benjamin K Chan,Scott A. Cunningham,Katherine E Goodman,Kerryl E. Greenwood-Quaintance,Anthony D. Harries,Shayla Hesse,Anthony W. Maresso,Veronique Nussenblatt,David T. Pride,Michael J. Rybak,Zoe Sund,David van Duin,Daria Van Tyne,Robin Patel +23 more
TL;DR: Suggestions are provided as answers to a series of questions intended to inform clinicians considering experimental phage therapy for patients in their clinical practices.
Journal ArticleDOI
Fast separation of large biomolecules using short monolithic columns.
TL;DR: The most efficient separation is expected when short monolithic column with accordingly adjusted gradient is implemented, especially for macromolecules interacting with the monolith functionalities via over 10 binding sites.
Journal ArticleDOI
CIM® monolithic anion-exchange chromatography as a useful alternative to CsCl gradient purification of bacteriophage particles
Evelien M. Adriaenssens,Susan M. Lehman,Katrien Vandersteegen,Dieter Vandenheuvel,Didier L. Philippe,Anneleen Cornelissen,Martha R. J. Clokie,Andrés J. García,Maurice De Proft,Martine Maes,Rob Lavigne +10 more
TL;DR: It is concluded that anion-exchange chromatography with monoliths is a valid alternative to the more traditional CsCl purification, has upscaling advantages, but it requires more extensive optimization.
Journal ArticleDOI
Principles and applications of steric exclusion chromatography
Jeremy Lee,Hui Theng Gan,Sarah Maria Abdul Latiff,Cindy Chuah,Wan Yee Lee,Yuansheng Yang,Bernard Loo,Say Kong Ng,Pete Gagnon +8 more
TL;DR: Hydroxyl-substituted polymethacrylate monoliths provide a hydrophilic surface and support convective mass transport that is unaffected by the viscosity of the PEG.
Journal ArticleDOI
Downstream processing and chromatography based analytical methods for production of vaccines, gene therapy vectors, and bacteriophages
TL;DR: This review gives an overview on thenanoplex downstream challenges and chromatography based analytical methods for efficient monitoring of the nanoplex production.
References
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Journal ArticleDOI
Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface
TL;DR: Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle that is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form.
Book
Bacteriophages: Biology and Applications
TL;DR: Bacteriophage as Antibiotics: Molecular Biology and Applications provides unparalleled, comprehensive information on bacteriophages and their applications, such as phage therapy, and covers the techniques, media, and methodology involved in isolating and working with therapeutic phages.
Journal ArticleDOI
Phage display: Concept, innovations, applications and future
TL;DR: The rapid isolation of specific ligands by phage display is advantageous in many applications including selection of inhibitors for the active and allosteric sites of the enzymes, receptor agonists and antagonists, and G-protein binding modulatory peptides.
Journal ArticleDOI
Bacteriophages and biotechnology: vaccines, gene therapy and antibacterials
Jason R. Clark,John B. March +1 more
TL;DR: It is hoped that the wide range of scientists, clinicians and biotechnologists currently researching or putting phages to practical use are able to pool their knowledge and expertise and thereby accelerate progress towards further development in this exciting field of biotechnology.
Book
Monolithic materials : preparation, properties and applications
TL;DR: Theoretical Aspects of Separation Using Short Monolithic Beds Monolithic Stationary Phases for the separation of Small Molecules Separation of Peptides and Proteins Nucleic Acid Analysis Synthetic Polymers Capillary Electrochromatography Large Scale Separations Immunoaffinity Assays Survey of Chromatographic and Electromigration Separations Miniature and Microchip Technologies Solid Phase Extraction Catalysts and Enzyme Reactors Solid Phase Synthesis and Auxiliaries for Combinatorial Chemistry as mentioned in this paper.