Showing papers in "Journal of Chromatography B in 2013"

TL;DR: The development of a LC-MS/MS-based method for simultaneous identification of endogenous concentrations of seven steroid hormones (cortisol, cortisone, testosterone, progesterone, corticosterone, dehydroepiandrosterone (DHEA) and androstenedione) in human hair is reported.

TL;DR: The variety of applied strategies for all "seven critical factors" in current literature on MS-based protein quantification have been critically reviewed and evaluated and the impact of strategies moving away from traditional protein cleavage isotope dilution mass spectrometry (PC-IDMS) toward approaches that are more dedicated to bioanalysis is predicted.

TL;DR: In this article, a simple methodology using liquid chromatography-tandem mass spectrometry allowing quantification of low-level PUFA metabolites including bioactive mediators, inactive products and pathway biomarkers was developed.

TL;DR: Doping control analyses are generally performed in urine, a matrix that provides a prolonged detection time window, and less often in blood, serum, plasma, hair, saliva, and nails, and to identify the chemical structures of anabolic steroids the use of mass spectrometry detection is very advantageous.

TL;DR: The aim of this work was to design, validate and apply a multi-component LC-MS/MS method suitable for screening of a large number of target analytes belonging to the class of new psychoactive substances - legal highs.

TL;DR: A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for measuring whole blood GSH and GSSG that minimizes preanalytic and analytic variability, reliably eliminates interference from ion suppression, and can easily be implemented in clinical laboratories is developed.

TL;DR: A simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to characterize the detailed BA profile in human urine and serum, indicating the role of sulfation in the detoxification of the more hydrophobic and toxic BA species.

TL;DR: A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, performed by electrospray ionization in positive mode using a triple quadrupole mass spectromaetry, has been developed and validated for the simultaneous determination of bortezomib (BORT), dasatinib (DASA), imatinIB (IMAT), nilotinib (NILO), erlotinib (ERLO), lapatinib

TL;DR: EME-DLLME-GC/FID is a promising combination for analysis of TCAs present at low concentrations in biological matrices for determination of tricyclic antidepressants (TCAs) in untreated human plasma and urine samples.

TL;DR: There was excellent correlation between the levels of tacrolimus and creatinine obtained from the clinical laboratory and the DBS method developed and this assay may have a significant impact on compliance with medication intake as well as potentially lowering the cost associated with intravenous blood draws in clinical laboratories.

TL;DR: An analytical method for measuring 12 biomarkers of several of these pesticides in urine that can be used to measure all the target analytes in one injection with similar repeatability and detection limits of previous methods which required more than one injection is developed.

TL;DR: A high performance liquid chromatography method with visible detection (HPLC-VIS) for the determination of malachite green (MG), crystal violet (CV), leucomalachitegreen (LMG), and leucocrystal violet (LCV) in fish has been developed after clean-up through an immunoaffinity column.

TL;DR: The most efficient separation is expected when short monolithic column with accordingly adjusted gradient is implemented, especially for macromolecules interacting with the monolith functionalities via over 10 binding sites.

TL;DR: The method developed for the quantitation of immunosuppressants in whole blood was used to analyze 276 patient samples containing tacrolimus and 55 samples containing cyclosporin A, and the results from LC/MS/MS were compared to those obtained from immunoassays of the same samples.

TL;DR: Monitoring of melanoma VOCs has potential as a useful screening methodology and analyses of the total volatile metabolome from both melanoma cells and normal melanocytes by DNACNT sensors, coupled with the GC-MS results, demonstrate clear differences between these cell systems.

TL;DR: Ultra-high performance liquid chromatography was applied for the first time in the analysis of wood extracts and the potential of this technique coupled to ion trap mass spectrometry in the rapid and effective detection and identification of bioactive components in complex vegetal samples was demonstrated.

TL;DR: The optimized procedure has been successfully applied to real samples and demonstrated that the new method was robust and useful for monitoring and quantification of 7 tetracycline residues in food of animal origin.

TL;DR: In this article, a high-performance liquid chromatographic method coupled with ultraviolet detection is presented for the simultaneous determination of human plasma concentration of six antiepileptic drugs frequently used in clinical practice.

TL;DR: A selective and sensitive high-performance liquid chromatography method for the concurrent determination of multiple antiepileptic drugs (AEDs) [levetiracetam (LVT), lamotrigine (LTG), phenobarbital (PHB)], carbamazepine (CBZ) and its active metabolite carbamazepsine-10,11 epoxide (CBZE)] in a single DBS has been developed and validated.

TL;DR: A rapid LC-MS/MS method was developed for simultaneous quantitation of 3 antifungals and one active metabolite and an antineoplastic agent in human plasma and is an efficient tool for routine TDM and optimization of laboratory resource utilization.

TL;DR: Molecular profiling and targeted analysis are considered, providing an overview of recent experimental or clinical works carried out using UHPLC analysis of compounds from various chemical classes, such as low molecular weight metabolites, hormones, lipids, peptides and proteins.

TL;DR: Two high-performance liquid chromatographic-electrospray ionization-mass spectrometric methods were developed and validated to estimate the melamine, bis(2-ethylhexyl) phthalate (DEHP), and bisphenol A (BPA) contents in drinking water, syringes, soft drinks, and dry milk powder.

TL;DR: A sensitive, sophisticated and practical bioanalytical assay for the simultaneous determination of six tyrosine kinase inhibitors and two active metabolites that can be used for guiding TKI therapy in daily clinical practice as well as for investigator-initiated research.

TL;DR: Profiling of polyunsaturated fatty acids (PUFAs) and their oxidized metabolites, mainly eicosanoids, in human plasma by fast liquid chromatography-mass spectrometry is described.

TL;DR: The proposed method was validated according to the European Commission Decision 2002/657/EC, which determines linearity, specificity, decision limit, detection capability, recovery, precision and stability.

TL;DR: Evaluating the success, at which the currently applied techniques have been utilized, and the evaluation provides directions in which future studies may be interesting to conduct in multi-steroid hormone procedures published from 2001 to 2012 is discussed.

TL;DR: The validated HPLC-MS/MS method was successfully applied to determination and comparative pharmacokinetics investigation of the ten constituents in rat plasma after oral administration of different combinations of Shaoyao-Gancao-Decoction (SGD) extracts.

TL;DR: There is certain relationship between exposure concentrations of phenolic environmental estrogens and uterine leiomyoma disease and BPA levels in experimental and control groups were not significantly different, but the mean levels and concentration distribution were different.

TL;DR: The results show that P. pastoris derived HBsAg VLPs exhibit a high potential as a superior biosimilar vaccine against hepatitis B.

TL;DR: Lyophilized feces demonstrated a more comprehensive metabolic coverage than fecal water, based on the number of chromatographic peaks, which highlights the importance of prudent interpretation of fecal metabonomic data, particularly in GIT diseases where bleeding is prevalent.