Showing papers in "Journal of Virological Methods in 2010"

TL;DR: These protocols should allow any laboratory knowledgeable in general virological and cell culture techniques to produce successfully replication-restricted rVSV-ΔG pseudotypes for subsequent analysis.

TL;DR: Three new real-time PCR assays based on TaqMan probe technology were reported, showing good specificity and sensitivity and will improve the rapid detection and differentiation of monkeypox infections from other rash illness.

TL;DR: HEV was shown to be present in European red deer for the first time and preselection based on species-independent serological assays may be beneficial to identify new potential animal reservoirs of HEV.

TL;DR: This assay and detection format represents the first step towards developing a practical, simple-to-use and inexpensive molecular assay format for ASF diagnosis in the field which is especially relevant to Africa where the disease is endemic in many countries.

TL;DR: It is demonstrated that ultracentrifugation is a more appropriate method for recovering RV-A from wastewater and that this method together with the multiplex qPCR assay may be suitable for routine laboratory use.

TL;DR: It has been concluded that serological results for goats by the different methods should be compared before final diagnosis because the specificity of methods in use can differ significantly.

TL;DR: The alternative protocol for quantitation of ostreid herpesvirus 1 in Pacific oysters Crassostrea gigas is an improvement of the reference protocol in terms of sensitivity, specificity and rapidity (<3h), according to the measures of Landis and Koch.

TL;DR: The PMA-PCR approach could be useful as a rapid and inexpensive analytical tool for screening and evaluation of the efficacy of disinfectants and for a more reliable understanding of the factors that contribute to viral inactivation through capsid damage monitoring.

TL;DR: Development of a real-time TaqMan® RT-PCR targeting the highly conserved nucleocapsid (N) gene of IBV and including an internal PCR control was described, allowing rapid and accurate IBV detection directly from clinical specimens, facilitating differential diagnosis.

TL;DR: It was attempted to stably transfect non-permissive PAM cells with CD163 cDNA to generate cell lines constitutively expressing CD163 and to evaluate their permissivity to PRRSV.

TL;DR: The two RT-PCR tests revealed consistently the presence of CBSV both in symptomatic and asymptomatic leaves and indicated that asymPTomatic leaves can be used reliably for virus diagnosis.

TL;DR: A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of pandemic influenza A H1N1 virus infection and demonstrated to be a sensitive and easy assay.

TL;DR: This method is a rapid and cost-effective alternative to existing serotype identification methods and offers a possibility to classify FAdV isolates more precisely, however, it has limitations such as need for extensive interpretation of results and potential for indeterminate results.

TL;DR: A one-step real-time Taqman RT-PCR assay for peste des petits ruminants virus (PPRV) was developed to detect the four lineages of PPRV by targeting the nucleoprotein (N) gene of the virus.

TL;DR: The real-time RT-PCR assay was found suitable for detection and epidemiological survey not only of Israeli H9N2 viruses, but also for isolates from other parts of the world.

TL;DR: The use of the recently developed ScriptCap post-transcriptional enzymatic capping system, followed by optimized Neon mediated electroporation of the highly permissive RAW 264.7 cells resulted in the rapid and robust recovery of infectious MNV.

TL;DR: The method described could be applied readily for viral biology studies and incorporated into proactive dengue virologic surveillance.

TL;DR: The results of this study demonstrate the potential application of hyperspectral remote sensing as a rapid, field-based method of identifying SCYLV-infected sugarcane plants prior to symptom expression.

TL;DR: This is the first report of CMV seed transmission in pepper and seed-growth tests in pots were performed and the rate of seed transmission was approximately 10 to 14%.

TL;DR: A viral entry assay where a beta-lactamase reporter protein fused to the influenza matrix protein-1 (BlaM1) is packaged as a structural component into influenza virus-like particles (VLPs), demonstrating that this straightforward assay has broad application for studying the entry steps of enveloped viruses, especially those that require high levels of biosafety containment.

TL;DR: Methacrylate monoliths (Convective Interaction Media monolithic columns) were applied for the purification of Staphylococcus aureus phages VDX-10 from bacterial lysate, resulting in more than 99% of host cell DNA and more than 90% of proteins were removed, with 60% recovery of viable phages.

TL;DR: A qualitative SYBR Green-based real-time multiplex RT-PCR for the simultaneous detection of hepatitis C virus (HCV) and human immunodeficiency virus type 1 (HIV-1) genomes in DBS may represent a reliable alternative for the detection of HIV-1/HCV co-infection, in rapid and relatively inexpensive screening programmes.

TL;DR: A multiplex polymerase chain reaction (PCR), that relies on a combination of previously published and newly designed primers was developed for the detection and identification of PVY(NTN-NW) and SYR-III in single or mixed infections with the main PVY strains.

TL;DR: A quantitative PCR assay was developed and used to determine the excretion level and concentration of bovine polyomaviruses in urine and environmental samples, including urban sewage, slaughterhouse sewage, and river water, which shows the spread of these viruses in many environmental samples containing contamination ofbovine origin.

TL;DR: Overall, the study shows that neither method was more biased than the other, and providing that an adequate number of PCR templates is analyzed, and that the bulk sequencing captures the diversity of the viral population, either method is likely to provide a similar measure of population diversity.

TL;DR: A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was established for the detection of nine viruses from infected rice plants, including rice black-streaked dwarf virus, which may facilitate studies on rice disease epidemiology, outbreak surveillance, and molecular pathology.

TL;DR: An affordable "in-house" genotyping assay to monitor HIV-1 drug resistance in Africa, particularly South Africa is developed and validated and is suited for use in Africa as it overcomes the obstacle of subtype diversity.

TL;DR: A broadly reactive TaqMan-based real-time reverse transcription (RT)-polymerase chain reaction (PCR) assay was developed for MNVs targeting highly conserved sequences among MNV strains, which are located in the open reading frames 1 (ORF1)-ORF2 junction region.

TL;DR: The lowest reliably determined virus concentration between, 5 and 10 KHV DNA genomic equivalents, was found by real-time PCR, nested PCR and one-tube semi-nested PCR, while all other published and unpublished PCRs, as well as the commercial Loopamp, recognized KHVDNA at higher concentrations only.

TL;DR: A novel, simple and easy to use approach, based on dried tube specimens (DTS), was developed that can help monitor the quality of HIV antibody testing in RLS and was piloted successfully in 24 testing sites in Kenya.