Characterization of a thermotolerant laccase produced by Streptomyces sp. SB086
Tatiana Alves Rigamonte Fernandes,Tatiana Alves Rigamonte Fernandes,Wendel Batista da Silveira,Flávia M. L. Passos,Tiago Domingues Zucchi +4 more
TLDR
The findings support in silico data of bacterial laccase secretion, and reinforce the view that actinomycetes may be a rich source of laccases for industrial application.Abstract:
Laccases have become desirable enzymes for application in many industrial processes. Nowadays, most of these enzymes are obtained from fungi. Among prospective studies for bacterial laccase genes, some have included actinomycetes, but only a few studies have characterized the enzyme produced. Thus, we have isolated a laccase-producing actinomycete from forest soil under restoration process and further aimed to characterize its produced enzyme. The isolate SB086 was assigned to the Streptomyces genus by a combination of phenotypical, chemical and phylogenetic properties. Our data indicate that the bacterium produces a thermotolerant laccase. The maximum activity was obtained in the pH range 4.0–5.0 and at 50 °C in reaction mixture containing 5 mM CuSO4; thermal stability was noted at 60 °C and 70 °C—a well-desired characteristic for industry. The active enzyme presented a high molecular mass (over 100 kDa) and was less sensitive to inhibition by metal ions than generally described for bacterial laccases. Our findings support in silico data of bacterial laccase secretion, and reinforce the view that actinomycetes may be a rich source of laccase for industrial application.read more
Citations
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Aptitude of Oxidative Enzymes for Treatment of Wastewater Pollutants: A Laccase Perspective
TL;DR: Seeing that both regulatory measures, as regards the discharge limits of wastewater, and the query for efficient treatment methods remain unanswered, partially, the prospects of enzymatic treatment of wastewater is advisable, and a reconsideration was assigned to the possible capacity of oxidative enzymes and the respective challenges encountered during their applications in wastewater treatment.
Journal ArticleDOI
Laccases from Actinobacteria —What We Have and What to Expect
Tatiana Alves Rigamonte Fernandes,Wendel Batista da Silveira,Flávia M. L. Passos,Tiago Domingues Zucchi +3 more
TL;DR: The relevant traits of actinomycetes laccases emphasize the need for more studies involving the isolation of this bacterial group from lignin-rich environmental samples, detection of their laccase activity and thereafter, characterization of the proteins and related genes.
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Lignocellulolytic systems of soil bacteria: A vast and diverse toolbox for biotechnological conversion processes.
TL;DR: Here, the lines of evidence that show the high potential of bacterial strains from soil for biomass conversion ranging from strain characterization to metagenome and metatranscriptome analysis are reviewed.
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Characterization of a thermo-alkali-stable laccase from Bacillus subtilis cjp3 and its application in dyes decolorization.
TL;DR: The purified recombinant laccase has a maximum activity of 7320 U/L, maintaining high stabilities under a wide pH range and high temperature conditions, and indicated the potential use of the CotA-laccase in treating wastewater containing synthetic dyes.
Journal ArticleDOI
Genomics and metatranscriptomics of biogeochemical cycling and degradation of lignin-derived aromatic compounds in thermal swamp sediment
David J. Levy-Booth,Ameena Hashimi,Raphael Roccor,Li-Yang Liu,Scott Renneckar,Lindsay D. Eltis,William W. Mohn +6 more
TL;DR: This study demonstrates how temperature structures biogeochemical cycling populations in a unique ecosystem, and combines community-level metagenomics with targeted metatranscriptomics to identify pathways with potential for bio-refinement of lignin-derived aromatic compounds.
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