Journal ArticleDOI
Characterization of complexes between recA protein and duplex DNA by electron microscopy
TLDR
The results suggest an intercalative mode of binding, which partially unwinds the DNA, in which 18.6 base-pairs and 6.4 recA monomers contribute to one turn of a right-handed helix.About:
This article is published in Journal of Molecular Biology.The article was published on 1982-05-05. It has received 216 citations till now. The article focuses on the topics: Scanning transmission electron microscopy & Negative stain.read more
Citations
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Journal ArticleDOI
Biochemistry of homologous recombination in Escherichia coli.
TL;DR: This review focuses on the biochemical mechanisms underlying these steps, with particular emphases on the activities of the proteins involved and on the integration of these activities into likely biochemical pathways for recombination.
Journal ArticleDOI
Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage λ
TL;DR: It is now appreciated that DNA repair and homologous recombination are related through DNA replication, and knowledge about recombinational repair in the broader context of DNA replication will guide future experimentation.
Journal ArticleDOI
The structure of the E. coli recA protein monomer and polymer.
TL;DR: The crystal structure of the recA protein from Escherichia coli at 2.3-A resolution reveals a major domain that binds ADP and probably single-and double-stranded DNA as discussed by the authors.
Journal ArticleDOI
Similarity of the yeast RAD51 filament to the bacterial RecA filament
TL;DR: The RAD51 protein functions in the processes of DNA repair and in mitotic and meiotic genetic recombination in the yeast Saccharomyces cerevisiae and has adenosine triphosphate-dependent DNA binding activities similar to those of the Escherichia coli RecA protein.
Journal ArticleDOI
Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA.
TL;DR: Using a topological assay, it is demonstrated that hRad51 underwinds duplex DNA, in a reaction dependent upon the presence of ATP or its non‐hydrolysable analogue ATP gamma S.A.
References
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Journal ArticleDOI
Reconstruction of Three Dimensional Structures from Electron Micrographs
D. J. De Rosier,Aaron Klug +1 more
TL;DR: General principles are formulated for the objective reconstruction of a three dimensional object from a set of electron microscope images and applied to the calculation of aThree dimensional density map of the tail of bacteriophage T4.
Journal ArticleDOI
Crystal structure analysis of a complete turn of B-DNA
Richard M. Wing,Richard M. Wing,Horace R. Drew,Tsunehiro Takano,Chris Broka,Shoji Tanaka,Keiichi Itakura,Richard E. Dickerson +7 more
TL;DR: This work reports the first single-crystal structure analysis of more than a complete turn of right-handed B-DNA, with the self-complementary dodecamer sequence d(CpGpCpC pGpApApTpTpCPGp CpG) or CGCGAATTCGCG.
Journal ArticleDOI
Calculation of binding isotherms for heterogeneous polymers.
TL;DR: The purpose of this paper is to point out that problems of this type are readily solved with good accuracy by use of random‐number methods on a high‐speed digital computer.
Journal ArticleDOI
E. coli recA protein-directed cleavage of phage lambda repressor requires polynucleotide.
TL;DR: It is suggested that a stoichiometric complex of recA protein and DNA is active both to destroy repressors by proteolytic cleavage and to initiate pairing of this DNA to its homologous sequence in a DNA duplex (‘strand invasion’).
Journal ArticleDOI
Helical periodicity of DNA determined by enzyme digestion
Daniela Rhodes,Aaron Klug +1 more
TL;DR: This work has found that the distance between successive DNase I cutting sites is 10.6 ± 0.1 bases for the DNA immobilized on three different surfaces, and identifies this value with the number of base pairs per turn of the DNA double helix in solution.