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Characterization of maize (Zea mays L.) Wee1 and its activity in developing endosperm (seedyreproductionyendoreduplicationycyclin-dependent kinase)
Yuejin Sun,Brian P. Dilkes,Chunsheng Zhang,Ricardo A. Dante,Newton Portilho Carneiro,Keith S. Lowe,R Udolf Jung,W Illiam J. Gordon-Kamm,Brian A. Larkins +8 more
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TLDR
In this article, a maize Wee1 homologue and its expression in developing endosperm was characterized using a 0.8-kb cDNA from an expressed sequence tag project, which encoded a protein of 403 aa with a calculated molecular size of 45.6 kDa.Abstract:
We report the characterization of a maize Wee1 homologue and its expression in developing endosperm. Using a 0.8-kb cDNA from an expressed sequence tag project, we isolated a 1.6-kb cDNA (ZmWee1), which encodes a protein of 403 aa with a calculated molecular size of 45.6 kDa. The deduced amino acid sequence shows 50% identity to the protein kinase domain of human Wee1. Overexpression of ZmWee1 in Schizosaccharomyces pombe inhibited cell division and caused the cells to enlarge significantly. Recombinant ZmWee1 obtained from Escherichia coli is able to inhibit the activity of p13 suc1 -adsorbed cyclin-dependent kinase from maize. ZmWee1 is encoded by a single gene at a locus on the long arm of chromosome 4. RNA gel blots showed the ZmWee1 transcript is about 2.4 kb in length and that its abundance reaches a maximum 15 days after pollination in endosperm tissue. High levels of expression of ZmWee1 at this stage of endosperm development imply that ZmWee1 plays a role in endoreduplication. Our results show that control of cyclin- dependent kinase activity by Wee1 is conserved among eu- karyotes, from fungi to animals and plants.read more
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Cell cycle regulation in plant development.
Dirk Inzé,Lieven De Veylder +1 more
TL;DR: The molecular mechanisms that regulate cell division and endoreduplication are reviewed and the understanding on how the cell cycle is integrated with plant development is discussed.
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Genome-Wide Analysis of Core Cell Cycle Genes in Arabidopsis
Klaas Vandepoele,Jeroen Raes,Jeroen Raes,Lieven De Veylder,Pierre Rouzé,Stephane Rombauts,Dirk Inzé +6 more
TL;DR: This genome-wide analysis illustrates the complexity of the plant cell cycle machinery and provides a tool for elucidating the function of new family members in the future.
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Knowing when to grow: signals regulating bud dormancy.
TL;DR: Epigenetic-like regulation of endodormancy suggests a possible role for chromatin remodeling similar to that known for the vernalization responses during flowering.
Journal ArticleDOI
The plant cell cycle.
TL;DR: This work reviews plant cell cycle regulators and their control and highlights the fundamental conservation of underlying cell cycle mechanisms between animals and plants.
Journal ArticleDOI
The Development of Endosperm in Grasses
TL;DR: The grass seed or caryopsis originates from a monocarpellary ovary with a single ovule and contains the main storage tissue, the endosperm, which determines the value of the crop.
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