Journal ArticleDOI
Characterization of Therapeutic Antibodies and Related Products
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Strategies and challenges for the next generation of antibody–drug conjugates
TL;DR: Strategies to select the best target antigens as well as suitable cytotoxic drugs; the design of optimized linkers; the discovery of bioorthogonal conjugation chemistries; and toxicity issues are discussed.
Journal ArticleDOI
Physicochemical characterization of Remsima
Soon Kwan Jung,Kyoung Hoon Lee,Jae Won Jeon,Joon Won Lee,Byoung Oh Kwon,Yeon Jung Kim,Jin Soo Bae,Dong-Il Kim,Soo Young Lee,Shin Jae Chang +9 more
TL;DR: Remsima® can be considered as a highly similar molecule to Remicade® in terms of physicochemical properties, efficacy, and safety for its final approval as a biosimilar product to Remiade®.
Journal ArticleDOI
MS-Based Allotype-Specific Analysis of Polyclonal IgG-Fc N-Glycosylation
Thomas Sénard,Andrea F.G. Gargano,Andrea F.G. Gargano,David Falck,Steven W. de Taeye,Theo Rispens,Gestur Vidarsson,Manfred Wuhrer,Govert W. Somsen,Elena Domínguez-Vega +9 more
TL;DR: This paper proposes a middle-up strategy for the analysis of the intact fragment crystallizable (Fc) region of human plasma IgGs, with the aim of acquiring integrated information of the N-glycosylation and other PTMs of subclasses and allotypes.
Journal ArticleDOI
Theory and practice of size exclusion chromatography for the analysis of protein aggregates.
TL;DR: This work discusses the commercialization of shorter and narrower columns packed with reduced particle sizes allowing an improvement in the resolution and throughput of SEC, and the possibility of combining SEC with various detectors, including refractive index (RI), ultraviolet (UV), multi-angle laser light scattering (MALLS) and viscometer (IV), for extensive characterization of protein samples.
References
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Journal ArticleDOI
Electrospray ionization for mass spectrometry of large biomolecules
TL;DR: Spectra have been obtained for biopolymers including oligonucleotides and proteins, the latter having molecular weights up to 130,000, with as yet no evidence of an upper limit.
Journal ArticleDOI
Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons
Michael Karas,Franz Hillenkamp +1 more
TL;DR: In the following, the first results on ultraviolet laser desorption (UVLD) of bioorganic compounds in the mass range above 10000 daltons are reported.
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Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization
TL;DR: The DESI phenomenon was observed both in the case of conductive and insulator surfaces and for compounds ranging from nonpolar small molecules such as lycopene, the alkaloid coniceine, and small drugs, through polar compounds such as peptides and proteins.
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Electrostatic Axially Harmonic Orbital Trapping: A High-Performance Technique of Mass Analysis
TL;DR: This work describes a new type of mass analyzer which employs trapping in an electrostatic field and demonstrates high-mass resolution up to 150,000 for ions produced by laser ablation, along with high-energy acceptance and wide mass range.
Journal ArticleDOI
Conjugation site modulates the in vivo stability and therapeutic activity of antibody-drug conjugates
Ben-Quan Shen,Keyang Xu,Luna Liu,Helga Raab,Sunil Bhakta,Margaret Kenrick,Kathryn L Parsons-Reponte,Janet Tien,Shang-Fan Yu,Elaine Mai,Dongwei Li,Jay Tibbitts,Jakub Baudys,Ola Saad,Suzie J. Scales,Paul J. Mcdonald,Philip E. Hass,Charles Eigenbrot,Trung Nguyen,Willy Solis,Reina N. Fuji,Kelly Flagella,Darshana Ramesh Patel,Susan D. Spencer,Leslie A. Khawli,Allen J. Ebens,Wai Lee Wong,Richard Vandlen,Surinder Kaur,Mark X. Sliwkowski,Richard H. Scheller,Paul Polakis,Jagath Reddy Junutula +32 more
TL;DR: In a mouse mammary tumor model, the stability and therapeutic activity of the antibody conjugate were affected positively by succinimide ring hydrolysis and negatively by maleimide exchange with thiol-reactive constituents in plasma.