Journal ArticleDOI
Chromosomal RNA: an artifact of preparation?
Michael Artman,Jay S. Roth +1 more
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TLDR
No evidence was found for the presence of a special class of RNA which is RNase resistant and covalently bound to chromosomal proteins, and Sucrose gradient sedimentation analysis of chromosonal RNA isolated under conditions minimizing RNA degradation showed it was comprised of all of the RNA species found in the nucleus.About:
This article is published in Journal of Molecular Biology.The article was published on 1971-09-14. It has received 60 citations till now. The article focuses on the topics: RNA & RNA-dependent RNA polymerase.read more
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Organization, transcription, and regulation in the animal genome.
Eric H. Davidson,Roy J. Britten +1 more
TL;DR: Recent experimental information in areas of animal cell molecular biology which are relevant to the mechanism of gene regulation are concerned, with particular reference to the frequency of structural gene sequences, mRNA turnover, and the interpretation of dipteran complementation groups.
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The Mechanism of Action of Androgens
TL;DR: A contemporary model for the Mechanism of Action of Androgen Action is presented, focusing on the Uptake, Retention, and Release of Androgens on Rat Ventral Prostate Gland and the Androgenic Regulation of RNA Synthesis: Receptor Effects in vitro.
Journal ArticleDOI
The non-histone proteins of chromatin, their isolation and composition in a number of tissues.
TL;DR: It is concluded that the non-histone proteins which remain tightly bound to DNA in chromatin are of the same approximate size as the basic histones.
Journal ArticleDOI
Primary Sequence of U-1 Nuclear Ribonucleic Acid of Novikoff Hepatoma Ascites Cells
TL;DR: The U-1 RNA is one of the low molecular weight nuclear RNAs of Novikoff hepatoma ascites cells that is specifically localized to the extranucleolar portion of the nucleus and is an alkali-stable tetranucleotide containing N2,2,7-trimethyl guanylic acid.
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Rat liver nuclear skeleton and small molecular weight RNA species.
T E Miller,C Y Huang,A O Pogo +2 more
TL;DR: Small molecular weight RNA species (smwRNAs) studied in rat liver nuclei with and without chromatin as well as with andwithout nuclear envelope and nucleoplasm have two unique properties: facility of extraction, and resistance to nuclear ribonuclease digestion.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Journal ArticleDOI
Gene Regulation for Higher Cells: A Theory
Roy J. Britten,Eric H. Davidson +1 more
TL;DR: Direct support for the idea that regulation of gene activity underlies cell differentiation comes from evidence that much of the genome in higher cell types is inactive and that different ribonucleic acids are synthesized in different cell types.
Journal ArticleDOI
Sequence-specific interaction of DNA and chromosomal protein.
TL;DR: It is shown that when chromosomal proteins, principally histones, are dissociated from DNA by 2 m -NaCl and then reassociated by gradient dialysis to low ionic strength, the original chromatin is not reconstituted, but reconstitution by gradientdialysis in the presence of 5 m -urea does result in chromatin equivalent to native by the hybridization-competition criterion.
Related Papers (5)
Effect of protein-bound RNA associated with chick embryo chromatin on template specificity of the chromatin☆
Ru Chih C. Huang,P.C. Huang +1 more