Journal ArticleDOI
Cloning and Sequence Analysis of the estA gene encoding enzyme for producing (R)-β-acetylmercaptoisobutyric acid from Pseudomonas aeruginosa 1001
TLDR
The estA gene encoding the enzyme that catalyzes the production of (R)-beta-acetylmercaptoisobutyric acid from (R,S)-ester from Pseudomonas aeruginosa 1001, was cloned in Escherichia coli and its nucleotide sequence was determined, revealing the presumed open reading frame encoding a polypeptide of 316 amino acid residues.About:
This article is published in Journal of Bioscience and Bioengineering.The article was published on 2000-01-01. It has received 10 citations till now. The article focuses on the topics: Peptide sequence & Consensus sequence.read more
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Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface
TL;DR: The lipase displayed on the Pichia pastoris cell surface exhibited better stability than the lipase LipB52 displayed on Saccharomyces cerevisiae cell surface, and the lipases exhibited similar transesterification activity, which makes them more suitable for whole-cell biocatalysts.
Journal ArticleDOI
Cloning and Characterization of a Novel Esterase from Rhodococcus sp. for Highly Enantioselective Synthesis of a Chiral Cilastatin Precursor
TL;DR: Fingerprinting analysis revealed that RhEst1 prefers para-nitrophenyl (pNP) esters of short-chain acyl groups, which could serve as an efficient hydrolase for the bioproduction of optically pure (S)-2,2-dimethyl cyclopropane carboxylic acid, which is an important chiral building block for cilastatin.
Journal ArticleDOI
A membrane-bound esterase PA2949 from Pseudomonas aeruginosa is expressed and purified from Escherichia coli.
Filip Kovacic,Florian Bleffert,Muttalip Caliskan,Susanne Wilhelm,Joachim Granzin,Renu Batra-Safferling,Karl-Erich Jaeger,Karl-Erich Jaeger +7 more
TL;DR: It is shown here that PA2949 from P. aeruginosa PA01, a homologue of EstA, can efficiently be expressed in an enzymatically active form in E. coli and has a conserved esterase catalytic triad consisting of Ser137–His258–Asp286.
Journal ArticleDOI
Cloning and expression of a novel esterase gene cpoA from Burkholderia cepacia.
TL;DR: Aims: To screen and clone a novel enzyme with specific activity for the resolution of (R)‐β‐acetylmercaptoisobutyrate (RAM) from (R,S)‐α‐sub 2,3,4,5‐triene‐2,6,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27
Journal ArticleDOI
Novel α-Amylase Inhibitor Hemi-Pyocyanin Produced by Microbial Conversion of Chitinous Discards
Thi Thanh Hanh Nguyen,San-Lang Wang,Anh Dzung Nguyen,Manh Dung Doan,Thi Ngoc Tran,Chien Thang Doan,Van Bon Nguyen +6 more
TL;DR: Bioactivity-guided purification resulted in the isolation of one major target compound, identified as hemi-pyocyanin (HPC) via gas chromatography-mass spectrometry and nuclear magnetic resonance, which demonstrated potent α-amylase inhibitory activity comparable to that of acarbose, a commercial antidiabetic drug.
References
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Design of specific inhibitors of angiotensin-converting enzyme: new class of orally active antihypertensive agents
TL;DR: A hypothetical model of the active site of angiotensin-converting enzyme, based on known chemical and kinetic properties of the enzyme, has enabled a new class of potent and specific inhibitors, carboxyalkanoyl and mercaptoalkanoysl derivatives of proline, to be designed.
Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
TL;DR: The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
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Translational Initiation in Prokaryotes
TL;DR: A conceptual framework for developing a theory of translational initiation and three approaches to a higher order approximation are described.
Journal ArticleDOI
Improvements in protein secondary structure prediction by an enhanced neural network
TL;DR: By adding neural network units that detect periodicities in the input sequence, this work modestly increased the secondary structure prediction accuracy, and the use of tertiary structural class causes a marked increase in accuracy.
Journal ArticleDOI
Characterization of translational initiation sites in E. coli.
TL;DR: The Shine and Dalgarno sequence of 124 known gene beginnings is characterized and this information is used to make "rules" which help distinguish gene beginning from other sites in a library of over 78,000 bases of mRNA.