Journal ArticleDOI
Cloning of nodule-specific cDNAs of Galega orientalis.
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TLDR
Differential display was applied in order to clone cDNAs expressed exclusively or predominantly in nodules, compared to uninoculated root tissue of Galega orientalis, which produced a nodule-specific signal on Northern hybridization.Abstract:
Differential display was applied in order to clone cDNAs expressed exclusively or predominantly in nodules, compared to uninoculated root tissue of Galega orientalis. Forty-five fragments were unique for nodule RNA. These fragments were reamplified and cloned. Six of them produced a nodule-specific signal on Northern hybridization. These six fragments were sequenced. Five of the sequenced fragments showed homology to nodulin-gene sequences in databases, among them Vicia faba mRNA for protein showing partial homology with Medicago sativa nodulin-25 (Nms25), Pisum sativum PsN466, V. faba CCP2 and CCP4, P. sativum ENOD3, and Maackia amurensis ENOD2. The remaining sequence had no significant homology with sequences in the databanks. Full-size cDNA for the homologue to V. faba mRNA for the protein showing partial homology with M. sativa nodulin-25 (Nms25) and P. sativum PsN466 were cloned and sequenced.read more
Citations
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Journal ArticleDOI
Computational Identification and Characterization of Novel Genes from Legumes
TL;DR: The Fabaceae, the third largest family of plants and the source of many crops, has been the target of many genomic studies, and evolutionary analyses of the genomic sequences of several CCPs in M. truncatula suggest that this family has evolved by local duplications and divergent selection.
Journal ArticleDOI
Loss of the nodule-specific cysteine rich peptide, NCR169, abolishes symbiotic nitrogen fixation in the Medicago truncatula dnf7 mutant
Beatrix Horvath,Ágota Domonkos,Attila Kereszt,Attila Szűcs,Edit Ábrahám,Ferhan Ayaydin,Károly Bóka,Yuhui Chen,Rujin Chen,Jeremy D. Murray,Michael K. Udvardi,Eva Kondorosi,Péter Kaló +12 more
TL;DR: It is demonstrated that the inability of M. truncatula dnf7 mutants to fix nitrogen is due to inactivation of a single NCR peptide, NCR169, which was induced in the cell layers in which bacteroid elongation was most pronounced, and high expression persisted throughout the nitrogen-fixing nodule zone.
Journal ArticleDOI
Genomic organization and evolutionary insights on GRP and NCR genes, two large nodule-specific gene families in Medicago truncatula.
Benoit Alunni,Zoltan Kevei,Miguel Redondo-Nieto,Miguel Redondo-Nieto,Adam Kondorosi,Peter Mergaert,Eva Kondorosi +6 more
TL;DR: Microsyntenic evidences between M. truncatula and L. japonicus validated the hypothesis that these genes are specific for the inverted repeat-lacking clade of hologalegoid legumes, which allowed dating the appearance of these two gene families during the evolution of legume plants.
Journal ArticleDOI
Plant and bacterial symbiotic mutants define three transcriptionally distinct stages in the development of the Medicago truncatula/Sinorhizobium meliloti symbiosis.
Raka M. Mitra,Sharon R. Long +1 more
TL;DR: Data suggest the presence of at least three transcriptionally distinct developmental stages during invasion of M. truncatula by S. meliloti and the regulation of these two genes and three previously identified genes in plant symbiotic mutants and wild-type plants inoculated with bacterial symbiosis mutants are examined.
Journal ArticleDOI
Morphotype of bacteroids in different legumes correlates with the number and type of symbiotic NCR peptides.
Jesús Montiel,J. Allan Downie,Attila Farkas,Péter Bihari,Róbert Herczeg,Balázs Bálint,Peter Mergaert,Attila Kereszt,Eva Kondorosi,Eva Kondorosi +9 more
TL;DR: The predicted NCR proteins in 10 legumes representing different subclades of the IRLC with distinct bacteroid morphotypes are identified and correlations between the composition of the NCR family and the morphotypes of bacteroids are established.
References
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Journal ArticleDOI
Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction
Peng Liang,Arthur B. Pardee +1 more
TL;DR: A method to separate and clone individual messenger RNAs (mRNAs) by means of the polymerase chain reaction using a set of oligonucleotide primers, one being anchored to the polyadenylate tail of a subset of mRNAs, the other being short and arbitrary in sequence so that it anneals at different positions relative to the first primer.
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A simple and efficient method for isolating RNA from pine trees
Journal ArticleDOI
Potential metal-binding domains in nucleic acid binding proteins.
TL;DR: Observations suggest numerous experiments are needed to determine whether metal-binding domains are present in proteins and, if present, what roles such domains play in the processes of nucleic acid binding and gene regulation.
Journal ArticleDOI
Sequential induction of Nodulin gene expression in the developing Pea Nodule
Ben Scheres,F.A. van Engelen,E. van der Knaap,C.C.M. van de Wiel,A. van Kammen,Ton Bisseling +5 more
TL;DR: The amino acid sequence derived from the nucleotide sequences of the cDNAs showed that ENOD5 is an arabinogalactan-like protein involved in the infection process, whereas ENOD3 and ENOD14 have a cysteine cluster suggesting that these are metal-binding proteins.
Journal ArticleDOI
Rhizobium galegae, a new species of legume root nodule bacteria
TL;DR: Studies of root nodule bacteria isolated from Galega orientalis and Galega officinalis are reviewed, and as a result a new species, Rhizobium galegae, is proposed, which forms nitrogen-fixing root nodules on G. Orientalis.