Close genetic linkage between human and companion animal extraintestinal pathogenic Escherichia coli ST127
Paarthiphan Elankumaran,Glenn F. Browning,Marc S. Marenda,Cameron J. Reid,Steven P. Djordjevic +4 more
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TLDR
This paper performed a phylogenomic analysis of 299 E. coli ST127 isolates of diverse epidemiological origin to characterize their population structure, genetic determinants of virulence, antimicrobial resistance, and repertoire of mobile genetic elements with a focus on plasmids.Abstract:
Escherichia coli ST127, a recently emerged global pathogen noted for high virulence gene carriage, is a leading cause of urinary tract and blood stream infections. ST127 is frequently isolated from humans and companion animals; however, it is unclear if they are distinct or related populations of ST127. We performed a phylogenomic analysis of 299 E. coli ST127 of diverse epidemiological origin to characterize their population structure, genetic determinants of virulence, antimicrobial resistance, and repertoire of mobile genetic elements with a focus on plasmids. The core gene phylogeny was divided into 13 clusters, the largest of which (BAP4) contained the majority of human and companion animal origin isolates. This dominant cluster displayed genetic differences to the remainder of the phylogeny, most notably alternative gene alleles encoding important virulence factors including lipid A, flagella, and K capsule. Furthermore, numerous close genetic linkages (<30 SNPs) between human and companion animal isolates were observed within the cluster. Carriage of antimicrobial resistance genes in the collection was limited, but virulence gene carriage was extensive. We found evidence of pUTI89-like virulence plasmid carriage in over a third of isolates, localised to four of the major phylogenetic clusters. Our study supports global scale repetitive transfer of E. coli ST127 lineages between humans and companion animals, particularly within the dominant BAP4 cluster. read more
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Genomic and Temporal Trends in Canine ExPEC Reflect Those of Human ExPEC
Paarthiphan Elankumaran,Max L. Cummins,Glenn F. Browning,Marc S. Marenda,Cameron J. Reid,Steven P. Djordjevic +5 more
TL;DR: It is found that most STs from canine infections are dominant human ExPEC STs with similar genomic traits, such as plasmid carriage and virulence gene burden, which are in support of anthropozoonosis.
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Clonal Dissemination of Extended-Spectrum Cephalosporin-Resistant Enterobacterales between Dogs and Humans in Households and Animal Shelters of Romania
TL;DR: A high prevalence of faecal ESC-R E. coli carriage in both dogs and humans from Romanian households and shelters is revealed, where bidirectional clonal transmission between humans and dogs is likely.
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Interspecies Transmission of CMY-2-Producing Escherichia coli Sequence Type 963 Isolates between Humans and Gulls in Australia
Matej Medvecky,Costas C. Papagiannitsis,Ethan R. Wyrsch,Ibrahim Bitar,Max L. Cummins,Steven P. Djordjevic,Monika Dolejska +6 more
TL;DR: Genomic analysis has revealed that ST963 is a globally dispersed lineage with a remarkable set of virulence genes and virulence plasmids described in uropathogenic E. coli.
Journal ArticleDOI
Global Phylogeny and F Virulence Plasmid Carriage in Pandemic Escherichia coli ST1193
TL;DR: In this article , a phylogenetic analysis of Escherichia coli ST1193 using publicly available sequences has been performed, with many examples sourced from human extraintestinal infections.
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An Emerging Lineage of Uropathogenic Extended Spectrum β-Lactamase Escherichia coli ST127
TL;DR: In this article , the authors reported the first whole-genome sequencing and analysis of ESBL UPEC ST127 isolates recovered from hospitalized patients in Armenia and compare them with other E. coli ST127 retrieved from the ENA.
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