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Journal ArticleDOI

Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation".

James J. McSharry, +1 more
- 01 Mar 1970 - 
- Vol. 40, Iss: 3, pp 745-746
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This article is published in Virology.The article was published on 1970-03-01. It has received 103 citations till now. The article focuses on the topics: Vesicular stomatitis virus.

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Citations
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Journal ArticleDOI

Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.

TL;DR: Extension of this method to concentration and purification of other viruses and nucleic acids, as well as some preliminary mechanistic studies, are discussed.
Journal ArticleDOI

Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples.

TL;DR: The polyethylene glycol method was shown to be more effective than the organic flocculation method for recovery of hepatitis A virus and rotaviruses Wa and SA11, but not of poliovirus 1 in laboratory studies.
Journal ArticleDOI

Isolation and Purification of the Envelope Proteins of Newcastle Disease Virus

TL;DR: Findings suggest that the association of hemagglutinating and neuraminidase activities with one glycoprotein is a general property of the paramyxovirus group.
Journal ArticleDOI

Size fractionation of double-stranded DNA by precipitation with polyethylene glycol.

TL;DR: PEG precipitation offers a size fractionation method for DNA which is convenient, of high capacity, and applicable over a wide range of conditions, however, resolution is not high and separation of two species approaches 100% only if they differ in molecular mass by at least a factor of two.
Journal ArticleDOI

Carbohydrate structure of vesicular stomatitis virus glycoprotein.

TL;DR: The results establish that the glycoprotein contains two identical carbohydrate chains that are composed of three outer chain units, a mannose-rich
References
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Journal ArticleDOI

Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.

TL;DR: Extension of this method to concentration and purification of other viruses and nucleic acids, as well as some preliminary mechanistic studies, are discussed.
Journal ArticleDOI

The fractionation of protein mixtures by linear polymers of high molecular weight.

TL;DR: Rapid simple methods for the preparation of γ-globulin and fibrinogen with the aid of polyethylene glycol are described and the products were homogeneous on electrophoresis and centrifugation and retained satisfactory biological properties.
Journal ArticleDOI

The isolation of plant viruses by means of "simple" coacervates.

TL;DR: The precipitation of viruses with polyethylene glycol is a rapid method for producing relatively pure virus and shown to have advantages over more conventional methods.
Journal Article

Biologic Properties of Two Plaque Variants of Vesicular Stomatitis Virus (Indiana Serotype)

TL;DR: Differences in infectivity, cytopathogenicity and growth potential were more pronounced when the two variants were compared in cultures of L cells, and definite evidence was obtained that the SP variant is more susceptible to the action of an exogenous interferon produced by infected L cells.
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