Confocal Brillouin microscopy for three-dimensional mechanical imaging
Giuliano Scarcelli,Seok Hyun Yun +1 more
TLDR
A confocal Brillouin microscope based on a fully parallel spectrometer-a virtually imaged phased array-that improves the detection efficiency by nearly 100-fold over previous approaches is demonstrated and the first cross-sectional BrillouIn imaging based on elastic properties as the contrast mechanism is shown.Abstract:
Acoustically induced inelastic light scattering, first reported in 1922 by Brillouin1, allows non-contact, direct readout of the viscoelastic properties of a material and has widely been investigated for material characterization2, structural monitoring3 and environmental sensing4. Extending the Brillouin technique from point sampling spectroscopy to imaging modality5 would open up new possibilities for mechanical imaging, but has been challenging because rapid spectrum acquisition is required. Here, we demonstrate a confocal Brillouin microscope based on a fully parallel spectrometer—a virtually imaged phased array—that improves the detection efficiency by nearly 100-fold over previous approaches. Using the system, we show the first cross-sectional Brillouin imaging based on elastic properties as the contrast mechanism and monitor fast dynamic changes in elastic modulus during polymer crosslinking. Furthermore, we report the first in situ biomechanical measurement of the crystalline lens in a mouse eye. These results suggest multiple applications of Brillouin microscopy in biomedical and biomaterial science.read more
Citations
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Micro‐/Nanostructured Mechanical Metamaterials
TL;DR: Various aspects of the micro-/nano-structured materials as mechanical metamaterials, potential tools for their multidimensional fabrication, and selected methods for their structural and performance characterization are described, as well as some prospects for the future developments in this exciting and emerging field.
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Non-reciprocal Brillouin scattering induced transparency
TL;DR: By exploiting the interaction between light and phonons in a silica microsphere resonator, it is possible to generate Brillouin scattering induced transparency, which is akin to electromagnetically induced transparency but for acoustic waves.
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Optical coherence elastography – OCT at work in tissue biomechanics [Invited]
Kirill V. Larin,David D. Sampson +1 more
TL;DR: The early developments in OCE, and the factors that led to the current acceleration, are described, with a strong emphasis on future prospects, which are exceptionally bright.
Journal ArticleDOI
Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy
Giuliano Scarcelli,Giuliano Scarcelli,William J. Polacheck,Hadi Tavakoli Nia,Kripa Patel,Alan J. Grodzinsky,Roger D. Kamm,Seok Hyun Yun,Seok Hyun Yun +8 more
TL;DR: The 3D Brillouin maps obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation.
Journal ArticleDOI
Brillouin optical microscopy for corneal biomechanics.
TL;DR: Brillouin imaging can assess the biomechanical properties of cornea in situ with high spatial resolution and has the potential for use in clinical diagnostics and treatment monitoring.
References
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Book
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Yuan-Cheng Fung,Richard Skalak +1 more
TL;DR: This chapter discusses the mechanics of Erythrocytes, Leukocytes, and Other Cells, and their role in Bone and Cartilage, and the properties of Bioviscoelastic Fluids, which are a by-product of these cells.
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Robert Langer,David A. Tirrell +1 more
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Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
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Journal Article
Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
TL;DR: In this article, a CARS-based multiphoton microscopy based on coherent anti-Stokes Raman scattering (CARS) was proposed for high-resolution 3D sectioning.