Construction of an Hfr strain useful for transferring recA mutations between Escherichia coli strains.
Laszlo N. Csonka,Alvin J. Clark +1 more
TLDR
Strain JC10240 (Hfr PO45 srlC300::Tn10 recA56 thr-300 ilv-318 rpsE300) was constructed and can be moved to other Escherichia coli strains in transductional or conjugational crosses selecting resistance to tetracycline.Abstract:
Strain JC10240 (Hfr PO45 srlC300::Tn10 recA56 thr-300 ilv-318 rpsE300) was constructed. On account of the close linkage of Tn10 to recA56, the latter can be moved to other Escherichia coli (and closely related) strains in transductional or conjugational crosses selecting resistance to tetracycline.read more
Citations
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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
Journal ArticleDOI
Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli.
TL;DR: It is reported here that E. coli contained an osmotically regulated trehalose-phosphate synthase which utilized UDP-glucose and glucose 6- phosphate as substrates and partially repressed the synthesis of synthase in the wild type and of beta-galactosidase in ots-lacZ fusion mutants.
Journal ArticleDOI
Bimodal pattern of killing of DNA-repair-defective or anoxically grown Escherichia coli by hydrogen peroxide.
James A. Imlay,Stuart Linn +1 more
TL;DR: Two modes of killing of Escherichia coli K-12 by hydrogen peroxide can be distinguished: mode-one killing required active metabolism during the H2O2 challenge, and it resulted in sfiA-independent filamentation of both cells which survived and those which were killed by the challenge; and mode-two killing did not require active metabolism, and killed cells did not filament, although survivors demonstrated a dose-dependent growth lag.
Journal ArticleDOI
ftsZ is an essential cell division gene in Escherichia coli.
Kang Dai,Joe Lutkenhaus +1 more
TL;DR: This strain was temperature sensitive for cell division and viability, confirming that ftsZ is an essential cell division gene in Escherichia coli and revealed that after a shift to the nonpermissive temperature, cell division ceased when the level of FtsZ started to decrease, indicating that septation is very sensitive to thelevel of FTSZ.
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Genetic analysis of the ompB locus in Escherichia coli K-12.
TL;DR: Fine-structure mapping and complementation analysis of the ompB locus indicate that this locus is comprised of two genes, envZ and ompR, which according to this model are a bifunctional regulatory protein and an envelope protein that senses the cell's external environment.
References
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Journal ArticleDOI
Recalibrated linkage map of Escherichia coli K-12.
B J Bachmann,K B Low,A L Taylor +2 more
TL;DR: This article corrects the article on p. 116 in vol.
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Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.
TL;DR: The results confirm that Tn10 often retains its physical and functional integrity during many cycles of translocation and gives rise to spontaneously occurring, tetracycline-sensitive deletions at high frequencies.
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Deletions Generated by the Transposon Tn10 in the srl recA Region of the ESCHERICHIA COLI K-12 Chromosome
Laszlo N. Csonka,Alvin J. Clark +1 more
TL;DR: A negative regulatory gene for the srl operon (srlR) was recognized by the characteristics of an insertion mutation generated by the transposon Tn10 determining tetracycline resistance.